Type

Journal Article

Authors

Sudha Srivastava
Richard O'Kennedy
Rahul Saxena
Julia Zapatero-Rodríguez
Shikha Sharma

Subjects

Chemistry

Topics
low cost modified electrodes gold nanoparticles human epidermal growth factor receptor 2 carbon electrodes screen printed electrodes electron transfer breast cancer

Ultrasensitive direct impedimetric immunosensor for detection of serum HER2. (2017)

Abstract Assesment of human epidermal growth factor receptor 2 status is a key factor prompting definitive treatment decisions that help in reducing mortality rates associated with breast cancer. In this article, highly sensitive and low-cost impedimetric immunosensor using single-chain fragment variable antibody fragments was developed for quantitative detection of human epidermal growth factor receptor 2 from serum employing gold nanoparticle-modified disposable screen-printed carbon electrodes. The gold nanoparticles facilitate fast electron transfer and offer a biocompatible surface for immobilization of small antibody fragments in an oriented manner, resulting in improved antigen binding efficiency. The single-chain fragment variable antibody fragment-modified screen printed immunosensor exhibits wide dynamic range of 0.01-100 ng mL
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Sudha Srivastava, Richard O'Kennedy, Rahul Saxena, Julia Zapatero-Rodríguez, Shikha Sharma

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Julia Zapatero-Rodríguez
Dublin City University
Total Publications: 6
 
3
Shikha Sharma
Dublin City University
Total Publications: 10