Type

Journal Article

Authors

Jan Tkac
Richard O'Kennedy
Julia Zapatero-Rodríguez
Pavel Damborsky
Stefan Belicky

Subjects

Chemistry

Topics
single chain antibodies recombinant antibody fragments prostate specific antigen chain length surface plasmon resonance prostate cancer fragments surface electrode

Full-length antibodies versus single-chain antibody fragments for a selective impedimetric lectin-based glycoprofiling of prostate specific antigen. (2017)

Abstract The main aim of the research was to design a functional impedimetric biosensor able to glycoprofile prostate specific antigen (PSA), a biomarker for prostate cancer (PCa), with high specificity using lectins as glycan recognising proteins. Traditionally, full-length antibody is immobilised on the biosensor interface for specific capture of PSA with subsequent glycoprofiling of PSA by addition of lectins. Since full-length antibodies contain glycans in the Fc domain, particular attention has to be paid to suppress direct binding of lectins to immobilised full-length antibodies, which would compromise accurate glycoprofiling. This issue is addressed here using a recombinant single-chain antibody fragments (scAb), which do not contain any carbohydrate moiety. Surface plasmon resonance was applied to prove negligible interaction of lectins with immobilised scAb fragments, while substantial binding of lectins to full length antibodies was observed. Eight different biosensor designs were tested for their ability to detect PSA. The biosensor device based on scAb fragments covalently immobilised on the gold electrode surface, patterned by a mixed SAM using standard amine coupling chemistry, proved to be the most sensitive. The scAb fragment-based biosensor exhibited sensitivity of 15.9 ± 0.8% decade
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Jan Tkac, Richard O'Kennedy, Julia Zapatero-Rodríguez, Pavel Damborsky, Stefan Belicky

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Julia Zapatero-Rodríguez
Dublin City University
Total Publications: 6