Type

Journal Article

Authors

Fergal J O'Brien
Garry P Duffy
Michael Walsh
Amos Matsiko
Alan Ryan
Amro Widaa
Tijna Alekseeva
Phoebe Roche

Subjects

Biochemistry

Topics
nerve guidance conduit stem cells rats sprague dawley cell growth nerve growth factor peripheral nerve conduit hyaluronic acid nerve regeneration

Olfactory Derived Stem Cells Delivered in a Biphasic Conduit Promote Peripheral Nerve Repair In Vivo. (2016)

Abstract Peripheral nerve injury presents significant therapeutic challenges for recovery of motor and sensory function in patients. Different clinical approaches exist but to date there has been no consensus on the most effective method of treatment. Here, we investigate a novel approach to peripheral nerve repair using olfactory derived stem (ONS) cells delivered in a biphasic collagen and laminin functionalized hyaluronic acid based nerve guidance conduit (NGC). Nerve regeneration was studied across a 10-mm sciatic nerve gap in Sprague Dawley rats. The effect of ONS cell loading of NGCs with or without nerve growth factor (NGF) supplementation on nerve repair was compared to a cell-free NGC across a variety of clinical, functional, electrophysiological, and morphologic parameters. Animals implanted with ONS cell loaded NGCs demonstrated improved clinical and electrophysiological outcomes compared to cell free NGC controls. The nerves regenerated across ONS cell loaded NGCs contained significantly more axons than cell-free NGCs. A return of the nocioceptive withdrawal reflex in ONS cell treated animals indicated an advanced repair stage at a relatively early time point of 8 weeks post implantation. The addition of NGF further improved the outcomes of the repair indicating the potential beneficial effect of a combined stem cell/growth factor treatment strategy delivered on NGCs. Stem Cells Translational Medicine 2017;6:1894-1904.
Collections Ireland -> National University of Ireland Galway -> PubMed

Full list of authors on original publication

Fergal J O'Brien, Garry P Duffy, Michael Walsh, Amos Matsiko, Alan Ryan, Amro Widaa, Tijna Alekseeva, Phoebe Roche

Experts in our system

1
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 268
 
2
Garry P Duffy
Royal College of Surgeons in Ireland
Total Publications: 59
 
3
Amos Matsiko
Royal College of Surgeons in Ireland
Total Publications: 24
 
4
Alan J Ryan
Royal College of Surgeons in Ireland
Total Publications: 7
 
5
Amro Widaa
Royal College of Surgeons in Ireland
Total Publications: 13
 
6
Phoebe Roche
Royal College of Surgeons in Ireland
Total Publications: 3