Type

Journal Article

Authors

Sinead Weldon
Clifford C Taggart
J Stuart Elborn
Catherine M Greene
Noel G McElvaney
Lauren Kerrigan
Sudha Priya Soundara Pandi
Qiwei Chen

Subjects

Biochemistry

Topics
cystic fibrosis fas cd95 cell lines cystic fibrosis transmembrane conductance regulator caspase 3 caspase 8 epithelial cells death receptors

Cystic fibrosis epithelial cells are primed for apoptosis as a result of increased Fas (CD95). (2017)

Abstract Previous work suggests that apoptosis is dysfunctional in cystic fibrosis (CF) airways with conflicting results. We evaluated the relationship between dysfunctional cystic fibrosis transmembrane conductance regulator (CFTR) and apoptosis in CF airway epithelial cells. Apoptosis and associated caspase activity were analysed in non-CF and CF tracheal and bronchial epithelial cell lines. Basal levels of apoptosis and activity of caspase-3 and caspase-8 were significantly increased in CF epithelial cells compared to controls, suggesting involvement of extrinsic apoptosis signalling, which is mediated by the activation of death receptors, such as Fas (CD95). Increased levels of Fas were observed in CF epithelial cells and bronchial brushings from CF patients compared to non-CF controls. Neutralisation of Fas significantly inhibited caspase-3 activity in CF epithelial cells compared to untreated cells. In addition, activation of Fas significantly increased caspase-3 activity and apoptosis in CF epithelial cells compared to control cells. Overall, these results suggest that CF airway epithelial cells are more sensitive to apoptosis via increased levels of Fas and subsequent activation of the Fas death receptor pathway, which may be associated with dysfunctional CFTR.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Sinead Weldon, Clifford C Taggart, J Stuart Elborn, Catherine M Greene, Noel G McElvaney, Lauren Kerrigan, Sudha Priya Soundara Pandi, Qiwei Chen

Experts in our system

1
Sinéad Weldon
Royal College of Surgeons in Ireland
Total Publications: 9
 
2
Clifford C Taggart
Royal College of Surgeons in Ireland
Total Publications: 54
 
3
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
4
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194