Type

Journal Article

Authors

Jochen H M Prehn
Paola Pizzo
Maria Ankarcrona
Daniele Bano
David S Park
Irene Llorente-Folch
Manuela Salvucci
Orla Watters
Catarina Moreira Pinho
Anna Gioran
and 36 others

Subjects

Microbiology

Topics
experimental models pathology guidelines and protocols cellular guidelines oxygen consumption neurodegenerative diseases mitochondrial diseases

Guidelines on experimental methods to assess mitochondrial dysfunction in cellular models of neurodegenerative diseases. (2017)

Abstract Neurodegenerative diseases are a spectrum of chronic, debilitating disorders characterised by the progressive degeneration and death of neurons. Mitochondrial dysfunction has been implicated in most neurodegenerative diseases, but in many instances it is unclear whether such dysfunction is a cause or an effect of the underlying pathology, and whether it represents a viable therapeutic target. It is therefore imperative to utilise and optimise cellular models and experimental techniques appropriate to determine the contribution of mitochondrial dysfunction to neurodegenerative disease phenotypes. In this consensus article, we collate details on and discuss pitfalls of existing experimental approaches to assess mitochondrial function in in vitro cellular models of neurodegenerative diseases, including specific protocols for the measurement of oxygen consumption rate in primary neuron cultures, and single-neuron, time-lapse fluorescence imaging of the mitochondrial membrane potential and mitochondrial NAD(P)H. As part of the Cellular Bioenergetics of Neurodegenerative Diseases (CeBioND) consortium ( www.cebiond.org ), we are performing cross-disease analyses to identify common and distinct molecular mechanisms involved in mitochondrial bioenergetic dysfunction in cellular models of Alzheimer's, Parkinson's, and Huntington's diseases. Here we provide detailed guidelines and protocols as standardised across the five collaborating laboratories of the CeBioND consortium, with additional contributions from other experts in the field.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Jochen H M Prehn, Paola Pizzo, Maria Ankarcrona, Daniele Bano, David S Park, Irene Llorente-Folch, Manuela Salvucci, Orla Watters, Catarina Moreira Pinho, Anna Gioran and 36 others

Experts in our system

1
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
2
Manuela Salvucci
Royal College of Surgeons in Ireland
 
3
Orla Watters
Royal College of Surgeons in Ireland
Total Publications: 5