Type

Journal Article

Authors

Daniel J Kelly
Rajalakshmanan Eswaramoorthy
Susan E Critchley

Subjects

Biochemistry

Topics
infrapatellar fat pad adult stem cells human cells stem cells co culture extracellular matrix cell proliferation tissue engineering

Low-oxygen conditions promote synergistic increases in chondrogenesis during co-culture of human osteoarthritic stem cells and chondrocytes. (2016)

Abstract There has been increased interest in co-cultures of stem cells and chondrocytes for cartilage tissue engineering as there are the limitations associated with using either cell type alone. Drawbacks associated with the use of chondrocytes include the limited numbers of cells available for isolation from damaged or diseased joints, their dedifferentiation during in vitro expansion, and a diminished capacity to synthesise cartilage-specific extracellular matrix components with age and disease. This has motivated the use of adult stem cells with either freshly isolated or culture-expanded chondrocytes for cartilage repair applications; however, the ideal combination of cells and environmental conditions for promoting robust chondrogenesis remains unclear. In this study, we compared the effect of combining a small number of freshly isolated or culture-expanded human chondrocytes with infrapatellar fat pad-derived stem cells (FPSCs) from osteoarthritic donors on chondrogenesis in altered oxygen (5% or 20%) and growth factor supplementation (TGF-β3 only or TGF-β3 and BMP-7) conditions. Both co-cultures, but particularly those including freshly isolated chondrocytes, were found to promote cell proliferation and enhanced matrix accumulation compared to the use of FPSCs alone, resulting in the development of a tissue that was compositionally more similar to that of the native articular cartilage. Local oxygen levels were found to impact chondrogenesis in co-cultures, with more robust increases in proteoglycan and collagen deposition observed at 5% O
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Daniel J Kelly, Rajalakshmanan Eswaramoorthy, Susan E Critchley

Experts in our system

1
Daniel Kelly
Trinity College Dublin
Total Publications: 168
 
2
Rajalakshmanan Eswaramoorthy
Royal College of Surgeons in Ireland
Total Publications: 3
 
3
Susan E Critchley
Royal College of Surgeons in Ireland
Total Publications: 3