Type

Journal Article

Authors

Sinead M. Waters
David A. Kenny
Alan K Kelly
Kate Keogh
Milka Popova
Paul Cormican
Matthew S. McCabe
Emily McGovern

Subjects

Agriculture & Food Science

Topics
relative abundance greenhouse gas emissions diversity anthropogenic greenhouse gas emissions cattle management ruminant livestock methane bovine rumen bovine rumen methanogens high throughput sequencing rumen nutrition compensatory growth

Plane of nutrition affects the phylogenetic diversity and relative abundance of transcriptionally active methanogens in the bovine rumen. (2017)

Abstract Methane generated during enteric fermentation in ruminant livestock species is a major contributor to global anthropogenic greenhouse gas emissions. A period of moderate feed restriction followed by ad libitum access to feed is widely applied in cattle management to exploit the animal's compensatory growth potential and reduce feed costs. In the present study, we utilised microbial RNA from rumen digesta samples to assess the phylogenetic diversity of transcriptionally active methanogens from feed-restricted and non-restricted animals. To determine the contribution of different rumen methanogens to methanogenesis during dietary restriction of cattle, we conducted high-throughput mcrA cDNA amplicon sequencing on an Illumina MiSeq and analysed both the abundance and phylogenetic origin of different mcrA cDNA sequences. When compared to their unrestricted contemporaries, in feed-restricted animals, the methanogenic activity, based on mcrA transcript abundance, of Methanobrevibacter gottschalkii clade increased while the methanogenic activity of the Methanobrevibacter ruminantium clade and members of the Methanomassiliicoccaceae family decreased. This study shows that the quantity of feed consumed can evoke large effects on the composition of methanogenically active species in the rumen of cattle. These data potentially have major implications for targeted CH
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Full list of authors on original publication

Sinead M. Waters, David A. Kenny, Alan K Kelly, Kate Keogh, Milka Popova, Paul Cormican, Matthew S. McCabe, Emily McGovern

Experts in our system

1
Sinead M. Waters
Teagasc
Total Publications: 99
 
2
D.A. Kenny
Teagasc
Total Publications: 147
 
3
Alan K Kelly
Teagasc
Total Publications: 88
 
4
Kate Keogh
Teagasc
Total Publications: 20
 
5
Paul Cormican
Teagasc
Total Publications: 54
 
6
Matthew S. McCabe
Teagasc
Total Publications: 45