Type

Journal Article

Authors

Cliona O'Farrelly
Elizabeth J Ryan
Justin G Geoghegan
Niamh Nolan
Emir Hoti
Donal Maguire
Aonghus Lavelle
Francesco Caiazza
Louise A Elliott
Cathal Harmon
and 1 others

Subjects

Biochemistry

Topics
metastasis cancer cells human cell migration il 6 inflammatory mediators liver leukocytes

Depleted polymorphonuclear leukocytes in human metastatic liver reflect an altered immune microenvironment associated with recurrent metastasis. (2017)

Abstract Hepatic immunity, normally protective against neoplasia, is subverted in colorectal liver metastasis (CRLM). Here, we compare the inflammatory microenvironment of CRLM-bearing liver tissue to donor liver. Twenty-five patients undergoing resection for CRLM were recruited, 13 of whom developed intrahepatic recurrence within 18 months. Biopsies were obtained from tumour and normal liver tissue adjacent to and distal from, the tumour. Donor liver biopsies were obtained during transplantation. Biopsies were cultured and conditioned media (CM) screened for 102 inflammatory mediators. Twelve of these were validated by Luminex assay. Transwell assays measured cancer cell chemotaxis. Polymorphonuclear leukocytes (PMN) and lymphocytes were quantified in H&E sections. Fewer periportal tissue-resident PMN were present in metastatic liver compared to donor liver. Patients with the fewest PMN in liver tissue distal to their tumour had a shorter time to intrahepatic recurrence (Pā€‰<ā€‰0.001). IL-6, CXCL1, CXCL5, G-CSF, GM-CSF, VEGF, LIF, and CCL3 were higher in liver-bearing CRLM compared to donor tissue. Consequently, cancer cells migrated equally towards CM of all regions of metastatic liver but not towards donor liver CM. The local inflammatory environment may affect both immune cell infiltration and cancer cell migration contributing to recurrence following resection for CRLM.
Collections Ireland -> Trinity College Dublin -> PubMed

Full list of authors on original publication

Cliona O'Farrelly, Elizabeth J Ryan, Justin G Geoghegan, Niamh Nolan, Emir Hoti, Donal Maguire, Aonghus Lavelle, Francesco Caiazza, Louise A Elliott, Cathal Harmon and 1 others

Experts in our system

1
Cliona O'Farrelly
Trinity College Dublin
Total Publications: 147
 
2
Elizabeth J Ryan
University College Dublin
 
3
Justin Geoghegan
Trinity College Dublin
Total Publications: 14
 
4
Emir Hoti
Trinity College Dublin
Total Publications: 4
 
5
Donal Maguire
Trinity College Dublin
Total Publications: 4
 
6
Aonghus Lavelle
University College Cork
Total Publications: 7
 
7
Francesco Caiazza
Trinity College Dublin
Total Publications: 4
 
8
Cathal Harmon
Trinity College Dublin
Total Publications: 9