Type

Journal Article

Authors

Jennifer Mahony
Douwe van Sinderen
Eoghan Casey

Subjects

Medicine & Nursing

Topics
real alternative therapies genomics biofilms life phage host interactions appropriate assessment lysins antibiotic resistance phages humans therapy phage preparation guide ph stability

In Vitro Characteristics of Phages to Guide 'Real Life' Phage Therapy Suitability. (2018)

Abstract The increasing problem of antibiotic-resistant pathogens has put enormous pressure on healthcare providers to reduce the application of antibiotics and to identify alternative therapies. Phages represent such an alternative with significant application potential, either on their own or in combination with antibiotics to enhance the effectiveness of traditional therapies. However, while phage therapy may offer exciting therapeutic opportunities, its evaluation for safe and appropriate use in humans needs to be guided initially by reliable and appropriate assessment techniques at the laboratory level. Here, we review the process of phage isolation and the application of individual pathogens or reference collections for the development of specific or "off-the-shelf" preparations. Furthermore, we evaluate current characterization approaches to assess the in vitro therapeutic potential of a phage including its spectrum of activity, genome characteristics, storage and administration requirements and effectiveness against biofilms. Lytic characteristics and the ability to overcome anti-phage systems are also covered. These attributes direct phage selection for their ultimate application as antimicrobial agents. We also discuss current pitfalls in this research area and propose that priority should be given to unify current phage characterization approaches.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> PubMed
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Jennifer Mahony, Douwe van Sinderen, Eoghan Casey

Experts in our system

1
Jennifer Mahony
University College Cork
Total Publications: 87
 
2
Douwe van Sinderen
University College Cork
Total Publications: 144
 
3
Eoghan Casey
University College Cork
Total Publications: 9