Type

Journal Article

Authors

Douwe van Sinderen
Marie-Pierre Chapot-Chartier
Jennifer Mahony
James Murphy
Thierry Grard
Irina Sadovskaya
Evgeny Vinogradov

Subjects

Agriculture & Food Science

Topics
structural species cell wall diversity lactococcus lactis nmr spectroscopy irish dairy receptors

Structural studies of the cell wall polysaccharide from Lactococcus lactis UC509.9. (2018)

Abstract Lactococcus lactis is the most widely utilised starter bacterial species in dairy fermentations. The L. lactis cell envelope contains polysaccharides, which, among other known functions, serve as bacteriophage receptors. Our previous studies have highlighted the structural diversity of these so-called cell wall polysaccharides (CWPSs) among L. lactis strains that could account for the narrow host range of most lactococcal bacteriophages. In the present work, we studied the CWPS of L. lactis strain UC509.9, an Irish dairy starter strain that is host to the temperate and well-characterized P335-type phage Tuc2009. The UC509.9 CWPS structure was analyzed by methylation, deacetylation/deamination, Smith degradation and 2D NMR spectroscopy. The CWPS consists of a linear backbone composed of a tetrasaccharide repeat unit, partially substituted with a branched phosphorylated oligosaccharide having a common trisaccharide and three non-stoichiometric substitutions.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Marie-Pierre Chapot-Chartier, Jennifer Mahony, James Murphy, Thierry Grard, Irina Sadovskaya, Evgeny Vinogradov

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Marie-Pierre Chapot-Chartier
University College Cork
 
3
Jennifer Mahony
University College Cork
Total Publications: 87
 
4
James Murphy
University College Cork
 
5
Irina Sadovskaya
University College Cork
Total Publications: 10
 
6
Evgeny Vinogradov
University College Cork