Type

Journal Article

Authors

Douwe van Sinderen
Jennifer Mahony
James Murphy
Philip Kelleher

Subjects

Biochemistry

Topics
dna methylation dna base detection technology real time sequencing next generation sequencing

Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology. (2017)

Abstract Whole phage genomes can be sequenced readily using one or a combination of next generation sequencing (NGS) technologies. One of the most recently developed NGS platforms, the so-called Single-Molecule Real-Time (SMRT) sequencing approach provided by the PacBio RS platform, is particularly useful in providing complete (i.e., un-gapped) genome sequences, but differs from other technologies in that the platform also allows for downstream analysis to identify nucleotides that have been modified by DNA methylation. Here, we describe the methodological approach for the detection of genomic methylation motifs by means of SMRT sequencing.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Jennifer Mahony, James Murphy, Philip Kelleher

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Jennifer Mahony
University College Cork
Total Publications: 87
 
3
James Murphy
University College Cork
 
4
Philip Kelleher
University College Cork