Type

Journal Article

Authors

David J Clarke
Julian R Marchesi
Ger McGlacken
Rachel Shanahan
Rafael Cano
Eoghan Casey
Elaine Crowley
Alli Lynch

Subjects

Biochemistry

Topics
human gut microbiome gut microbiota escherichia coli environmental dna fragilis hemolysins eubacterium coprostanoligenes functional analysis glycine bacterium metabolism biosynthesis bacteroidales expression bacteroides fragilis cholesterol lipids

The Bacteroidales produce an N-acylated derivative of glycine with both cholesterol-solubilising and hemolytic activity. (2017)

Abstract The contribution of the gut microbiota to the metabolism of cholesterol is not well understood. In this study, we identify 21 fosmid clones from a human gut microbiome metagenomic library that, when expressed in Escherichia coli, produce halos on LB agar supplemented with 0.01% (w/v) cholesterol (LBC agar). Analysis of 14 of these clones revealed that they all share a fragment of DNA with homology to the genome of Bacteroides vulgatus. The gene responsible for halo production on LBC agar, named choA, was identified as an N-acyltransferase known to produce an acylated glycine molecule called commendamide. In this study we show that commendamide is capable of producing a halo on LBC agar suggesting that this molecule is solubilizing the cholesterol micelles in LBC agar. We also show that commendamide is responsible for the previously described hemolytic activity associated with the choA orthologue in Bacteroides fragilis. A functional analysis of ChoA identified 2 amino acids that are important for commendamide biosynthesis and we present phylogenetic and functional data showing that orthologues of choA are found only in the order Bacteroidales. Therefore, the production of commendamide may be an adaptation to the environments colonized by the Bacteroidales, including the mammalian gut.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> PubMed
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Chemistry
Ireland -> University College Cork -> Chemistry - Journal Articles
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

David J Clarke, Julian R Marchesi, Ger McGlacken, Rachel Shanahan, Rafael Cano, Eoghan Casey, Elaine Crowley, Alli Lynch

Experts in our system

1
David J. Clarke
University College Cork
Total Publications: 24
 
2
Julian R Marchesi
University College Cork
Total Publications: 45
 
3
Rachel Shanahan
University College Cork
Total Publications: 10
 
4
Rafael Cano
University College Cork
Total Publications: 7
 
5
Eoghan Casey
University College Cork
Total Publications: 9