Type

Journal Article

Authors

Christian Cambillau
Douwe van Sinderen
Marco Ventura
Gabriele A. Lugli
Laurie Ramond
Arjen Nauta
Jennifer Mahony
Renaud Vincentelli
Stephen Hayes

Subjects

Biochemistry

Topics
phage host interactions distal tail protein binding proteins bacteriophage receptor binding protein gram positive bacteria carbohydrate binding module lactococcus lactis

Functional carbohydrate binding modules identified in evolved Dits from siphophages infecting various Gram-positive bacteria (2018)

Abstract With increasing numbers of 3D structures of bacteriophage components, combined with powerful in silico predictive tools, it has become possible to decipher the structural assembly and associated functionality of phage adhesion devices. Recently, decorations have been reported in the tail and neck passage structures of members of the so‐called 936 group of lactococcal siphophages. In the current report, using bioinformatic analysis we identified a conserved carbohydrate binding module (CBM) among many of the virion baseplate Dit components, in addition to the CBM present in the ‘classical’ receptor binding proteins (RBPs). We observed that, within these so‐called ‘evolved’ Dit proteins, the identified CBMs have structurally conserved folds, yet can be grouped into four distinct classes. We expressed such modules in fusion with GFP, and demonstrated their binding capability to their specific host using fluorescent binding assays with confocal microscopy. We detected evolved Dits in several phages infecting various Gram‐positive bacterial species, including mycobacteria. The omnipresence of CBM domains in Siphophages indicates their auxiliary role in infection, as they can assist in the specific recognition of and attachment to their host, thus ensuring a highly efficient and specific phage‐host adhesion process as a prelude to DNA injection.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
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Full list of authors on original publication

Christian Cambillau, Douwe van Sinderen, Marco Ventura, Gabriele A. Lugli, Laurie Ramond, Arjen Nauta, Jennifer Mahony, Renaud Vincentelli, Stephen Hayes

Experts in our system

1
Christian Cambillau
University College Cork
Total Publications: 18
 
2
Douwe van Sinderen
University College Cork
Total Publications: 144
 
3
Marco Ventura
University College Cork
Total Publications: 52
 
4
Gabriele Andrea Lugli
University College Cork
Total Publications: 35
 
5
Arjen Nauta
University College Cork
Total Publications: 11
 
6
Jennifer Mahony
University College Cork
Total Publications: 87
 
7
Stephen Hayes
University College Cork
Total Publications: 7