Type

Journal Article

Authors

Malcolm R. Smyth
Robert J Forster
Richard O'Kennedy
Anthony J. Killard
Máire B. O'Connor

Subjects

Chemistry

Topics
biochemistry ru ii complexes chemistry polypyridyl osmium ii biotechnology ruthenium ii antibody

Production and characterization of a polyclonal antibody fornext term Os(II) and Ru(II) polypyridyl complexes (2006)

Abstract The characterization of a polyclonal antibody produced via immunization with an [Os(bpy)2dcbpy] hapten is described. Bpy is 2,2′-bipyridine and dcbpy is 2,2′-bipyridine-4,4′-dicarboxylic acid. The cross-reactivity of the antibody for the Ru(II) analogue of the hapten was also investigated. Large increases in the emission and luminescent lifetime of a series of Os and Ru complexes were observed on binding of the antibody. Association equilibrium constants were derived from luminescence titration data and were found to be 5.6 × 108 and 5.0 × 108 M−1 for [Os(bpy)2dcbpy] and [Ru(bpy)2dcbpy], respectively. Spectroscopic changes were likely due to the exclusion of H2O from the complex/antibody binding cleft and blocking of vibrational relaxation pathways of the Os/Ru excited state. D2O/H2O experiments confirmed that the antibody protected approx. 82% of [Os(bpy)2dcbpy] and 80% of [Ru(bpy)2dcbpy] from excited state deactivation by the aqueous solvent.
Collections Ireland -> Dublin City University -> Publication Type = Article
Ireland -> Dublin City University -> Subject = Physical Sciences: Chemistry
Ireland -> Dublin City University -> Status = Published
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Chemical Sciences
Ireland -> Dublin City University -> Subject = Biological Sciences: Biochemistry
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: National Centre for Sensor Research (NCSR)
Ireland -> Dublin City University -> Subject = Biological Sciences: Biotechnology
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Biotechnology

Full list of authors on original publication

Malcolm R. Smyth, Robert J Forster, Richard O'Kennedy, Anthony J. Killard, Máire B. O'Connor

Experts in our system

1
Robert J. Forster
Dublin City University
Total Publications: 90
 
2
R O'Kennedy
Dublin City University
Total Publications: 197
 
3
Anthony J. Killard
Dublin City University
Total Publications: 73