Type

Journal Article

Authors

Richard O'Kennedy
Paul Leonard
Stephen J. Daly
Anthony J. Killard
Paul P. Dillon

Subjects

Chemistry

Topics
chemistry biosensor surface plasmon resonance morphine 3 glucuronide inhibition assay plasmon resonance spr biosensors biotechnology

Novel assay format permitting the prolonged usenext term of regeneration-based sensor chip technology (2004)

Abstract A polyclonal antibody raised against morphine-3-glucuronide (M3G, the main metabolite of heroin and morphine) was used in the development of a novel assay format using a surface plasmon resonance (SPR)-based biosensor. Previously developed assays have generated calibration curves based on differences in the quantity of response units binding to the surface of a chip coated with the analyte. The novel assay described here was based on the development of a standard curve using the slope of a series of consecutive binding interactions. Using this format, regeneration between each assay cycle was no longer required. This increased the useable life span of the chip surface and, as a result, decreased the cost associated with the assay. Thus, at least 15 binding interactions could be carried out before the saturation of antibody on the surface of the chip caused the response to deviate significantly from linearity. After 15 nonregenerated binding interactions, the slope still remained within 1.5% of the slope after a single binding event. Analysis time, and the sample volumes required were also markedly decreased while sensitivity was enhanced. The inhibition assay developed had a detection range of 270 to 17,500 pg ml 1 .
Collections Ireland -> Dublin City University -> Publication Type = Article
Ireland -> Dublin City University -> Subject = Physical Sciences: Chemistry
Ireland -> Dublin City University -> Status = Published
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Chemical Sciences
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: National Centre for Sensor Research (NCSR)
Ireland -> Dublin City University -> Subject = Biological Sciences: Biotechnology
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Biotechnology
Ireland -> Dublin City University -> Subject = Biological Sciences: Biosensors

Full list of authors on original publication

Richard O'Kennedy, Paul Leonard, Stephen J. Daly, Anthony J. Killard, Paul P. Dillon

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Paul Leonard
Dublin City University
Total Publications: 27
 
3
Anthony J. Killard
Dublin City University
Total Publications: 73