Type

Journal Article

Authors

Richard Murphy
Karina Horgan
Martin Clynes
Paula Meleady
Indre Sinkunaite
Padraig Doolan
Laura Breen
Michael Henry
Finbarr O'Sullivan
Joanne Keenan

Subjects

Chemistry

Topics
reactive oxygen species caco 2 sources cell lines copper compounds metal binding ubiquitinated proteins unfolded protein response

Acute exposure to organic and inorganic sources of copper: Differential response in intestinal cell lines. (2018)

Abstract Copper supplementation in nutrition has evolved from using inorganic mineral salts to organically chelated minerals but with limited knowledge of the impact at the cellular level. Here, the impact of inorganic and organic nutrient forms (glycinate, organic acid, and proteinate) of copper on the cellular level is investigated on intestinal cell lines, HT29 and Caco-2, after a 2-hr acute exposure to copper compounds and following a 10-hr recovery. Following the 10-hr recovery, increases were observed in proteins involved in metal binding (metallothioneins) and antioxidant response (sulfiredoxin 1 and heme oxygenase 1), and global proteomic analysis suggested recruitment of the unfolded protein response and proteosomal overloading. Copper organic acid chelate, the only treatment to show striking and sustained reactive oxygen species generation, had the greatest impact on ubiquitinated proteins, reduced autophagy, and increased aggresome formation, reducing growth in both cell lines. The least effect was noted in copper proteinate with negligible impact on aggresome formation or extended growth for either cell line. The type and source of copper can impact significantly at the cellular level.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Richard Murphy, Karina Horgan, Martin Clynes, Paula Meleady, Indre Sinkunaite, Padraig Doolan, Laura Breen, Michael Henry, Finbarr O'Sullivan, Joanne Keenan

Experts in our system

1
Richard Murphy
Dublin City University
 
2
Karina Horgan
Dublin City University
Total Publications: 6
 
3
Martin Clynes
Dublin City University
Total Publications: 232
 
4
Paula Meleady
Dublin City University
Total Publications: 115
 
5
Padraig Doolan
Dublin City University
Total Publications: 38
 
6
Laura Breen
Dublin City University
 
7
Michael Henry
Dublin City University
Total Publications: 99
 
8
Finbarr O'Sullivan
Dublin City University
Total Publications: 24
 
9
Joanne Keenan
Dublin City University
Total Publications: 27