Type

Journal Article

Authors

Finbarr O'Sullivan
Richard Murphy
Patrick Ward
Indre Sinkunaite
Martin Clynes
Karina Horgan
Joanne Keenan

Subjects

Biochemistry

Topics
chemistry humans culture medium culture media caco 2 cells cell culture techniques trace elements

Unexpected fluctuations of trace element levels in cell culture medium in vitro: caveat emptor. (2018)

Abstract Copper is an essential trace element micronutrient in human and animal nutrition. Trace amounts present even in ultrapure water, serum and other cell culture medium components are sufficient to support the health requirements of most cell types in culture. Analysis of a variety of different types of basal media from a number of different suppliers revealed large fluctuations in the levels of copper, and also of other micronutrients including zinc, iron, selenium and cobalt. Investigations on proliferating Caco-2 cells revealed reductions in growth with increasing copper concentrations within the range seen in the commercial media and changes in expression of apoptosis- and autophagy-related proteins were noted. Even at concentrations of 1 μM CuSO
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Finbarr O'Sullivan, Richard Murphy, Patrick Ward, Indre Sinkunaite, Martin Clynes, Karina Horgan, Joanne Keenan

Experts in our system

1
Finbarr O'Sullivan
Dublin City University
Total Publications: 24
 
2
Richard Murphy
Dublin City University
 
3
Martin Clynes
Dublin City University
Total Publications: 232
 
4
Karina Horgan
Dublin City University
Total Publications: 6
 
5
Joanne Keenan
Dublin City University
Total Publications: 27