Journal Article


Luke A.J. O'Neill
Caroline Jefferies
Sinead Miggin
Claudia Wietek



kinase 2 interferon regulatory factor 4 transcription factor nf b interferon regulatory factor 3 gene expression toll like receptor receptor activation

Interferon Regulatory Factor-3-mediated Activation of the Interferon-sensitive Response Element by Toll-like receptor (TLR) 4 but Not TLR3 Requires the p65 Subunit of NF-κB (2003)

Abstract Interferon regulatory factor (IRF) 3 is a transcription factor that binds the interferon-sensitive response element (ISRE) and is activated by Toll-like receptor 3 (TLR3) and TLR4. We have found that a dominant negative form of I κB kinase 2 and a mutant form of Iκ B, which acts as a super-repressor of NFκ B, blocked activation of the ISRE by the TLR4 ligand lipopolysaccharide but not the TLR3 ligand poly(I-C). TLR4 failed to activate the ISRE in mouse embryonic fibroblasts bearing a targeted deletion of p65, whereas the response to TLR3 in these cells was normal. The p65 subunit of NFκ B was detected in the lipopolysaccharide-activated but not poly(I-C)-activated ISRE-binding complex. Finally, p65 promoted transactivation of gene expression by IRF-3. These results therefore indicate that IRF-3mediated activation of the ISRE by TLR4 but not TLR3 requires the p65 subunit of NFκB.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Status = Published

Full list of authors on original publication

Luke A.J. O'Neill, Caroline Jefferies, Sinead Miggin, Claudia Wietek

Experts in our system

Luke Anthony John O'Neill
Trinity College Dublin
Total Publications: 262
Caroline A Jefferies
Royal College of Surgeons in Ireland
Total Publications: 50
Sinead M Miggin
Maynooth University
Total Publications: 31