Type

Journal Article

Authors

Paul Dowling
Ross Morgan
Martin Clynes
Vincent Lynch
Michael Emmet O'Brien
Abduladim Hmmier

Subjects

Medicine & Nursing

Topics
lung carcinoma mass spectrometry cancer patients lung cancer cancer diagnosis carcinoma squamous cell non small cell lung cancer nsclc bronchoalveolar lavage fluid

Proteomic analysis of bronchoalveolar lavage fluid (BALF) from lung cancer patients using label-free mass spectrometry (2017)

Abstract Background: Lung cancer is the leading cause of cancer-related mortality in both men and women throughout the world. The need to detect lung cancer at an early, potentially curable stage, is essential and may reduce mortality by 20%. The aim of this study was to identify distinct proteomic profiles in bronchoalveolar fluid (BALF) and plasma that are able to discriminate individuals with benign disease from those with non-small cell lung cancer (NSCLC). Methods: Using label-free mass spectrometry analysis of BALF during discovery-phase analysis, a significant number of proteins were found to have different abundance levels when comparing control to adenocarcinoma (AD) or squamous cell lung carcinoma (SqCC). Validation of candidate biomarkers identified in BALF was performed in a larger cohort of plasma samples by detection with enzyme-linked immunoassay. Results: Four proteins (Cystatin-C, TIMP-1, Lipocalin-2 and HSP70/HSPA1A) were selected as a representative group from discovery phase mass spectrometry BALF analysis. Plasma levels of TIMP-1, Lipocalin-2 and Cystatin-C were found to be significantly elevated in AD and SqCC compared to control. Conclusion: The results presented in this study indicate that BALF is an important proximal biofluid for the discovery and identification of candidate lung cancer biomarkers. General significance: There is good correlation between the trend of protein abundance levels in BALF and that of plasma which validates this approach to develop a blood biomarker to aid lung cancer diagnosis, particularly in the era of lung cancer screening. The protein signatures identified also provide insight into the molecular mechanisms associated with lung malignancy.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Research Institutes: Human Health Institute
Ireland -> Maynooth University -> Status = Published

Full list of authors on original publication

Paul Dowling, Ross Morgan, Martin Clynes, Vincent Lynch, Michael Emmet O'Brien, Abduladim Hmmier

Experts in our system

1
Paul Dowling
Maynooth University
Total Publications: 82
 
2
Ross Morgan
Dublin City University
Total Publications: 3
 
3
Martin Clynes
Dublin City University
Total Publications: 232
 
4
Vincent Lynch
Dublin City University
Total Publications: 12
 
5
M Emmet O'Brien
Royal College of Surgeons in Ireland
Total Publications: 9
 
6
Abduladim Hmmier
Maynooth University
Total Publications: 4