Type

Journal Article

Authors

Kay Ohlendieck
Dieter Swandulla
Rustam R Mundegar
Paula Meleady
Michael Henry
Margit Zweyer
Paul Dowling
Sandra Murphy

Subjects

Biochemistry

Topics
mass spectrometry inflammation plasma inflammatory response tissue damage muscular dystrophy duchenne animal model membrane protein

Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy (2017)

Abstract X-linked muscular dystrophy is caused by primary abnormalities in the Dmd gene and is characterized by the almost complete loss of the membrane cytoskeletal protein dystrophin, which triggers sarcolemmal instability, abnormal calcium homeostasis, increased proteolysis and impaired excitation‑contraction coupling. In addition to progressive necrosis, crucial secondary pathologies are represented by myofibrosis and the invasion of immune cells in damaged muscle fibres. In order to determine whether these substantial changes within the skeletal musculature are reflected by an altered rate of protein release into the circulatory system or other plasma fluctuations, we used label‑free mass spectrometry to characterize serum from the mdx‑4cv model of Duchenne muscular dystrophy. Comparative proteomics revealed a large number of increased vs. decreased protein species in mdx‑4cv serum. A serum component with greatly elevated levels was identified as the inflammation‑inducible plasma marker haptoglobin. This acute phase response protein is usually secreted in relation to tissue damage and sterile inflammation. Both immunoblot analyses and enzyme‑linked immunosorbent assays confirmed the increased concentration of haptoglobin in crude mdx‑4cv serum. This suggests that haptoglobin, in conjunction with other altered serum proteins, represents a novel diagnostic, prognostic and/or therapy‑monitoring biomarker candidate to evaluate the inflammatory response in the mdx‑4cv animal model of dystrophinopathy.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Research Institutes: Human Health Institute
Ireland -> Maynooth University -> Status = Published

Full list of authors on original publication

Kay Ohlendieck, Dieter Swandulla, Rustam R Mundegar, Paula Meleady, Michael Henry, Margit Zweyer, Paul Dowling, Sandra Murphy

Experts in our system

1
Kay Ohlendieck
Maynooth University
Total Publications: 131
 
2
Dieter Swandulla
Maynooth University
Total Publications: 26
 
3
Rustam R Mundegar
Maynooth University
Total Publications: 17
 
4
Paula Meleady
Dublin City University
Total Publications: 115
 
5
Michael Henry
Dublin City University
Total Publications: 99
 
6
Margit Zweyer
Maynooth University
Total Publications: 24
 
7
Paul Dowling
Maynooth University
Total Publications: 82
 
8
Sandra Murphy
Maynooth University
Total Publications: 32