Type

Journal Article

Authors

Marina A Lynch
Brian A. Lawlor
Deirdre Lohan
Laura Williams
Chris McGuigan
Anne-Marie Miller
Aedín M Minogue
Virginia Mela Rivas
Hannah Wolfe
Marina Lynch

Subjects

Biochemistry

Topics
human monocytes j774 2 cells thp 1 cells neuroscience glycolysis mci alzheimer s disease ageing inflammatory markers plasma app ps1 mice

Monocytes exposed to plasma from patients with Alzheimer's disease undergo metabolic reprogramming (2019)

Abstract The search for a blood-based biomarker that identifies Alzheimer’s disease (AD) and can replace current invasive and expensive diagnostic tests, continues. The most extensively-examined peripheral marker is β-amyloid (Aβ) but the results are inconsistent across studies and do not reflect the changes that take place in the brain. Several studies have assessed possible proteomic signatures but with inconsistent findings, although increases in circulating inflammatory molecules are generally observed. Here, rather than focus on identifying changes in the circulation, we evaluated the effect of plasma from patients with mild cognitive impairment (MCI) and AD on the human monocyte-like cell line, THP-1 cells, and plasma from an AD mouse model on a mouse monocyte-macrophage cell line, J774.2 cells. Plasma from AD patients and the AD mouse model increased inflammatory molecules in the cells and these changes were accompanied by an increase in glycolysis. Interestingly, plasma from MCI patients exerted no significant effect on THP-1 cells. The possibility therefore exists that evaluating the effect of plasma on IL-8 and TNFα mRNA in THP-1 cells combined with analysis of glycolysis in these cells, may be the basis of an indicator that discriminates between AD and MCI and normal controls, but is unlikely to be useful in identifying early pathological changes.
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Full list of authors on original publication

Marina A Lynch, Brian A. Lawlor, Deirdre Lohan, Laura Williams, Chris McGuigan, Anne-Marie Miller, Aedín M Minogue, Virginia Mela Rivas, Hannah Wolfe, Marina Lynch

Experts in our system

1
Marina Annetta Lynch
Trinity College Dublin
Total Publications: 80
 
2
Brian A. Lawlor
National College Ireland
Total Publications: 125
 
3
Laura Williams
Trinity College Dublin
Total Publications: 18
 
4
Chris McGuigan
Trinity College Dublin
Total Publications: 5
 
5
Anne-Marie Miller
Trinity College Dublin
Total Publications: 6
 
6
Aedín M Minogue
Trinity College Dublin
Total Publications: 10
 
7
Hannah Wolfe
National College Ireland
Total Publications: 7