Type

Journal Article

Authors

Douwe van Sinderen
Arjen Nauta
Jennifer Mahony
Christian Cambillau
Charles M. A. P. Franz
Jean-Paul Noben
James Murphy
Horst Neve
Yoan Duhoo
Stephen Hayes

Subjects

Veterinary

Topics
receptor virus protein binding group dairy processing host interactions lactic acid bacteria

Identification of dual receptor binding protein systems in Lactococcal 936 group phages (2018)

Abstract Siphoviridae of the lactococcal 936 group are the most commonly encountered bacteriophages in the dairy processing environment. The 936 group phages possess a discrete baseplate at the tip of their tail—a complex harbouring the Receptor Binding Protein (RBP) which is responsible for host recognition and attachment. The baseplate-encoding region is highly conserved amongst 936 phages, with 112 of 115 publicly available phages exhibiting complete synteny. Here, we detail the three exceptions (Phi4.2, Phi4R15L, and Phi4R16L), which differ from this genomic architecture in possessing an apparent second RBP-encoding gene upstream of the “classical” rbp gene. The newly identified RBP possesses an elongated neck region relative to currently defined 936 phage RBPs and is genetically distinct from defined 936 group RBPs. Through detailed characterisation of the representative phage Phi4.2 using a wide range of complementary techniques, we demonstrated that the above-mentioned three phages possess a complex and atypical baseplate structure. Furthermore, the presence of both RBPs in the tail tip of the mature virion was confirmed, while the anticipated host-binding capabilities of both proteins were also verified.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Douwe van Sinderen, Arjen Nauta, Jennifer Mahony, Christian Cambillau, Charles M. A. P. Franz, Jean-Paul Noben, James Murphy, Horst Neve, Yoan Duhoo, Stephen Hayes

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Arjen Nauta
University College Cork
Total Publications: 11
 
3
Jennifer Mahony
University College Cork
Total Publications: 87
 
4
Christian Cambillau
University College Cork
Total Publications: 18
 
5
Charles M. A. P. Franz
University College Cork
 
6
Jean-Paul Noben
University College Cork
Total Publications: 13
 
7
James Murphy
University College Cork
 
8
Horst Neve
University College Cork
Total Publications: 41
 
9
Stephen Hayes
University College Cork
Total Publications: 7