Type

Journal Article

Authors

Julian R Marchesi
Cormac G M Gahan
Ciarán O'Brien
Eileen Broaders

Subjects

Environment

Topics
microbiome osmotolerance potassium chloride health and wellbeing gut mobile genetic elements salt tolerance human

Evidence for plasmid-mediated salt tolerance in the human gut microbiome and potential mechanisms (2016)

Abstract The human gut microbiome is critical to health and wellbeing. It hosts a complex ecosystem comprising a multitude of bacterial species, which contributes functionality that would otherwise be absent from the host. Transient and commensal bacteria in the gut must withstand many stresses. The influence of mobile genetic elements such as plasmids in stress adaptation within the ecosystem is poorly understood. Using a mobilomic approach we found evidence for plasmid-mediated osmotolerance as a phenotype amongst the Proteobacteria in healthy faecal slurries. A transconjugant carrying multiple plasmids acquired from healthy faecal slurry demonstrated increased osmotolerance in the presence of metal salts, particularly potassium chloride, which was not evident in the recipient. Pyrosequencing and analysis of the total plasmid DNA demonstrated the presence of plasmid-borne osmotolerance systems (including KdpD and H-NS) which may be linked to the observed phenotype. This is the first report of a transferable osmotolerance phenotype in gut commensals and may have implications for the transfer of osmotolerance in other niches.
Collections Ireland -> University College Cork -> College of Science, Engineering and Food Science
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Ireland -> University College Cork -> Microbiology - Journal Articles
Ireland -> University College Cork -> Pharmacy - Journal Articles

Full list of authors on original publication

Julian R Marchesi, Cormac G M Gahan, Ciarán O'Brien, Eileen Broaders

Experts in our system

1
Julian R Marchesi
University College Cork
Total Publications: 45
 
2
Cormac G M Gahan
University College Cork
Total Publications: 124