Type

Journal Article

Authors

Jennifer Mahony
Douwe van Sinderen
Marco Ventura
Gabriele A. Lugli
Jean-Paul Noben
Charles M. A. P. Franz
Horst Neve
Eoghan Casey

Subjects

Veterinary

Topics
phage therapy enterotoxigenic escherichia coli shigella ssp escherichia coli bacterial infections bacteriophage developing countries host range

A quest of great importance-developing a broad spectrum Escherichia coli phage collection (2019)

Abstract Shigella ssp. and enterotoxigenic Escherichia coli are the most common etiological agents of diarrheal diseases in malnourished children under five years of age in developing countries. The ever-growing issue of antibiotic resistance and the potential negative impact of antibiotic use on infant commensal microbiota are significant challenges to current therapeutic approaches. Bacteriophages (or phages) represent an alternative treatment that can be used to treat specific bacterial infections. In the present study, we screened water samples from both environmental and industrial sources for phages capable of infecting E. coli laboratory strains within our collection. Nineteen phages were isolatedand tested for their ability to infect strains within the ECOR collection and E. coli O157:H7 Δstx. Furthermore, since coliphages have been reported to cross-infect certain Shigella spp., we also evaluated the ability of the nineteen phages to infect a representative Shigella sonnei strain from our collection. Based on having distinct (although overlapping in some cases) host ranges, ten phage isolates were selected for genome sequence and morphological characterization. Together, these ten selected phages were shown to infect most of the ECOR library, with 61 of the 72 strains infected by at least one phage from our collection. Genome analysis of the ten phages allowed classification into five previously described genetic subgroups plus one previously underrepresented subgroup.
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Full list of authors on original publication

Jennifer Mahony, Douwe van Sinderen, Marco Ventura, Gabriele A. Lugli, Jean-Paul Noben, Charles M. A. P. Franz, Horst Neve, Eoghan Casey

Experts in our system

1
Jennifer Mahony
University College Cork
Total Publications: 87
 
2
Douwe van Sinderen
University College Cork
Total Publications: 144
 
3
Marco Ventura
University College Cork
Total Publications: 52
 
4
Gabriele Andrea Lugli
University College Cork
Total Publications: 35
 
5
Jean-Paul Noben
University College Cork
Total Publications: 13
 
6
Charles M. A. P. Franz
University College Cork
 
7
Horst Neve
University College Cork
Total Publications: 41
 
8
Eoghan Casey
University College Cork
Total Publications: 9