Type

Journal Article

Authors

Christian Cambillau
Douwe van Sinderen
Arjen Nauta
Laurie Ramond
Renaud Vincentelli
Jennifer Mahony
Stephen Hayes

Subjects

Biochemistry

Topics
bacteriophage lactococcus lactis receptor binding protein carbohydrate binding module green fluorescent proteins modules phage host interactions protein structure

Ubiquitous carbohydrate binding modules decorate 936 lactococcal siphophage virions (2019)

Abstract With the availability of an increasing number of 3D structures of bacteriophage components, combined with powerful in silico predictive tools, it has become possible to decipher the structural assembly and functionality of phage adhesion devices. In the current study, we examined 113 members of the 936 group of lactococcal siphophages, and identified a number of Carbohydrate Binding Modules (CBMs) in the neck passage structure and major tail protein, on top of evolved Dit proteins, as recently reported by us. The binding ability of such CBM-containing proteins was assessed through the construction of green fluorescent protein fusion proteins and subsequent binding assays. Two CBMs, one from the phage tail and another from the neck, demonstrated definite binding to their phage-specific host. Bioinformatic analysis of the structural proteins of 936 phages reveals that they incorporate binding modules which exhibit structural homology to those found in other lactococcal phage groups and beyond, indicating that phages utilize common structural “bricks” to enhance host binding capabilities. The omnipresence of CBMs in Siphophages supports their beneficial role in the infection process, as they can be combined in various ways to form appendages with different shapes and functionalities, ensuring their success in host detection in their respective ecological niches.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Christian Cambillau, Douwe van Sinderen, Arjen Nauta, Laurie Ramond, Renaud Vincentelli, Jennifer Mahony, Stephen Hayes

Experts in our system

1
Christian Cambillau
University College Cork
Total Publications: 18
 
2
Douwe van Sinderen
University College Cork
Total Publications: 144
 
3
Arjen Nauta
University College Cork
Total Publications: 11
 
4
Jennifer Mahony
University College Cork
Total Publications: 87
 
5
Stephen Hayes
University College Cork
Total Publications: 7