Type

Journal Article

Authors

Douwe van Sinderen
Jennifer Mahony
Graham F. Hatfull
Marco Ventura
Horst Neve
Gabriele Andrea Lugli
Charles M. A. P. Franz
Kieran James
Francesca Bottacini
Joana Oliveira
and 2 others

Subjects

Veterinary

Topics
bifidobacterium longum protein binding actinobacteriophages infectious particles induction bifidobacterial prophages human gut bifidobacterium breve

Characterization and induction of prophages in human gut-associated Bifidobacterium hosts (2018)

Abstract In the current report, we describe the identification of three genetically distinct groups of prophages integrated into three different chromosomal sites of human gut-associated Bifidobacterium breve and Bifidobacterium longum strains. These bifidobacterial prophages are distantly related to temperate actinobacteriophages of several hosts. Some prophages, integrated within the dnaJ2 gene, are competent for induction, excision, replication, assembly and lysis, suggesting that they are fully functional and can generate infectious particles, even though permissive hosts have not yet been identified. Interestingly, several of these phages harbor a putative phase variation shufflon (the Rin system) that generates variation of the tail-associated receptor binding protein (RBP). Unlike the analogous coliphage-associated shufflon Min, or simpler Cin and Gin inversion systems, Rin is predicted to use a tyrosine recombinase to promote inversion, the first reported phage-encoded tyrosine-family DNA invertase. The identification of bifidobacterial prophages with RBP diversification systems that are competent for assembly and lysis, yet fail to propagate lytically under laboratory conditions, suggests dynamic evolution of bifidobacteria and their phages in the human gut.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Douwe van Sinderen, Jennifer Mahony, Graham F. Hatfull, Marco Ventura, Horst Neve, Gabriele Andrea Lugli, Charles M. A. P. Franz, Kieran James, Francesca Bottacini, Joana Oliveira and 2 others

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Jennifer Mahony
University College Cork
Total Publications: 87
 
3
Marco Ventura
University College Cork
Total Publications: 52
 
4
Horst Neve
University College Cork
Total Publications: 41
 
5
Gabriele Andrea Lugli
University College Cork
Total Publications: 35
 
6
Charles M. A. P. Franz
University College Cork
 
7
Kieran James
University College Cork
Total Publications: 7
 
8
Francesca Bottacini
University College Cork
Total Publications: 32
 
9
Joana Oliveira
University College Cork
Total Publications: 7