Type

Journal Article

Authors

Douwe van Sinderen
Jan-Maarten A. Geertman
Tadhg O'Sullivan
Richard John Roberts
Philip Kelleher
Jennifer Mahony
Marine Feyereisen

Subjects

Agriculture & Food Science

Topics
beer spoilage pan genome oxidation reduction lactobacillus brevis lactic acid bacteria smrt sequencing beer adaptation genomics

Comparative genome analysis of the Lactobacillus brevis species (2019)

Abstract Background: Lactobacillus brevis is a member of the lactic acid bacteria (LAB), and strains of L. brevis have been isolated from silage, as well as from fermented cabbage and other fermented foods. However, this bacterium is also commonly associated with bacterial spoilage of beer. Results: In the current study, complete genome sequences of six isolated L. brevis strains were determined. Five of these L. brevis strains were isolated from beer (three isolates) or the brewing environment (two isolates), and were characterized as beer-spoilers or non-beer spoilers, respectively, while the sixth isolate had previously been isolated from silage. The genomic features of 19‚ÄČL. brevis strains, encompassing the six L. brevis strains described in this study and thirteen L. brevis strains for which complete genome sequences were available in public databases, were analyzed with particular attention to evolutionary aspects and adaptation to beer. Conclusions: Comparative genomic analysis highlighted evolution of the taxon allowing niche colonization, notably adaptation to the beer environment, with approximately 50 chromosomal genes acquired by L. brevis beer-spoiler strains representing approximately 2% of their total chromosomal genetic content. These genes primarily encode proteins that are putatively involved in oxidation-reduction reactions, transcription regulation or membrane transport, functions that may be crucial to survive the harsh conditions associated with beer. The study emphasized the role of plasmids in beer spoilage with a number of unique genes identified among L. brevis beer-spoiler strains.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Douwe van Sinderen, Jan-Maarten A. Geertman, Tadhg O'Sullivan, Richard John Roberts, Philip Kelleher, Jennifer Mahony, Marine Feyereisen

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Tadhg O'Sullivan
University College Cork
Total Publications: 7
 
3
Philip Kelleher
University College Cork
 
4
Jennifer Mahony
University College Cork
Total Publications: 87
 
5
Marine Feyereisen
University College Cork
Total Publications: 6