Type

Journal Article

Authors

Douwe van Sinderen
Marco Ventura
Gabriele A. Lugli
Francesca Bottacini
Jennifer Mahony
Philip Kelleher

Subjects

Agriculture & Food Science

Topics
conjugation lactic acid bacteria genome sequencing lactococcus lactis plasmid smrt sequencing lactococcal dairy fermentation

The Lactococcus lactis pan-plasmidome (2019)

Abstract Plasmids are autonomous, self-replicating, extrachromosomal genetic elements that are typically not essential for growth of their host. They may encode metabolic capabilities, which promote the maintenance of these genetic elements, and may allow adaption to specific ecological niches and consequently enhance survival. Genome sequencing of 16 Lactococcus lactis strains revealed the presence of 83 plasmids, including two megaplasmids. The limitations of Pacific Biosciences SMRT sequencing in detecting the total plasmid complement of lactococcal strains is examined, while a combined Illumina/SMRT sequencing approach is proposed to combat these issues. Comparative genome analysis of these plasmid sequences combined with other publicly available plasmid sequence data allowed the definition of the lactococcal plasmidome, and facilitated an investigation into (bio)technologically important plasmid-encoded traits such as conjugation, bacteriocin production, exopolysaccharide (EPS) production and (bacterio)phage resistance.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Douwe van Sinderen, Marco Ventura, Gabriele A. Lugli, Francesca Bottacini, Jennifer Mahony, Philip Kelleher

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Marco Ventura
University College Cork
Total Publications: 52
 
3
Gabriele Andrea Lugli
University College Cork
Total Publications: 35
 
4
Francesca Bottacini
University College Cork
Total Publications: 32
 
5
Jennifer Mahony
University College Cork
Total Publications: 87
 
6
Philip Kelleher
University College Cork