Type

Journal Article

Authors

Douwe van Sinderen
Jennifer Mahony
Thijs Kouwen
Emiel Ver Loren van Themaat
Evgeny Vinogradov
Irina Sadovskaya
Katherine Lavelle
Philip Kelleher
Francesca Bottacini
Laurens Hanemaaijer
and 1 others

Subjects

Chemistry

Topics
cell wall polysaccharide mutation nmr nmr spectroscopy dairy adsorption

A cell wall-associated polysaccharide is required for bacteriophage adsorption to the Streptococcus thermophilus cell surface (2020)

Abstract Streptococcus thermophilus strain ST64987 was exposed to a member of a recently discovered group of S. thermophilus phages (the 987 phage group), generating phage-insensitive mutants, which were then characterized phenotypically and genomically. Decreased phage adsorption was observed in selected bacteriophage-insensitive mutants, and was partnered with a sedimenting phenotype and increased cell chain length or aggregation. Whole genome sequencing of several bacteriophage-insensitive mutants identified mutations located in a gene cluster presumed to be responsible for cell wall polysaccharide production in this strain. Analysis of cell surface-associated glycans by methylation and NMR spectroscopy revealed a complex branched rhamno-polysaccharide in both ST64987 and phage-insensitive mutant BIM3. In addition, a second cell wall-associated polysaccharide of ST64987, composed of hexasaccharide branched repeating units containing galactose and glucose, was absent in the cell wall of mutant BIM3. Genetic complementation of three phage-resistant mutants was shown to restore the carbohydrate and phage resistance profiles of the wild-type strain, establishing the role of this gene cluster in cell wall polysaccharide production and phage adsorption and, thus, infection.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Research Institutes and Centres

Full list of authors on original publication

Douwe van Sinderen, Jennifer Mahony, Thijs Kouwen, Emiel Ver Loren van Themaat, Evgeny Vinogradov, Irina Sadovskaya, Katherine Lavelle, Philip Kelleher, Francesca Bottacini, Laurens Hanemaaijer and 1 others

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Jennifer Mahony
University College Cork
Total Publications: 87
 
3
Thijs R. Kouwen
University College Cork
Total Publications: 10
 
4
Evgeny Vinogradov
University College Cork
 
5
Irina Sadovskaya
University College Cork
Total Publications: 10
 
6
Philip Kelleher
University College Cork
 
7
Francesca Bottacini
University College Cork
Total Publications: 32
 
8
Laurens Hanemaaijer
University College Cork
Total Publications: 10