Type

Journal Article

Authors

Lorraine Brennan
Michael J Gibney
Anne P Nugent
Breige A McNulty
Milena Rundle
Helena Gibbons
Xiaofei Yin

Subjects

Agriculture & Food Science

Topics
data analysis food intake metabolomics 3 methylhistidine estimated chicken intake guanidoacetate dietary markers national nutrition survey

Estimation of chicken intake using metabolomics derived markers (2017)

Abstract Background: Improved assessment of meat intake using metabolomics derived markers can provide objective data and could be helpful in clarifying proposed associations between meat intake and health.Objective: The objective was to identify novel markers of chicken intake using a metabolomics approach, and use markers to determine intake in an independent cohort. Methods: Ten participants (age, 62 y; BMI, 28.25 Kg/m2) in NutriTech Food Intake Study (NCT01684917) consumed increased amounts of chicken from 88 to 290 g/day over three weeks. Urine and blood samples were analyzed by NMR and MS, respectively. Multivariate data analysis was performed to identify markers associated with chicken intake. A calibration curve was built based on dose response association using NutriTech data. Bland and Altman analysis evaluated the agreement between reported and calculated chicken intake in National Adult Nutrition Survey (NANS) cohort. Results: Multivariate data analysis of postprandial and fasting urine samples collected in NutriTech revealed good discrimination between high (290 g/day) and low (88 g/day)  chicken intakes. Urinary metabolite profiles showed differences in metabolite levels between low and high chicken intakes. Examining metabolite profiles revealed guanidoacetate significantly increased from 1.47 to 3.66 mmol/L following increasing chicken intake from 88 to 290 g/day (P < 0.01). Using a calibration curve developed from NutriTech study, chicken intake was calculated in NANS, where chicken consumers had higher guanidoacetate excretion (0.70 mmol/L) than non-consumers (0.47 mmol/L) (P < 0.01). Bland and Altman analysis revealed good agreement between reported and calculated intakes with a bias of -30.2g/day. Plasma metabolite analysis demonstrated that 3-methylhistidine (3-Meth-His) was a more suitable indicator of chicken intake compared with 1-methylhistidine (1-Meth-His). Conclusions: Guanidoacetate was successfully identified and confirmed as a marker of chicken intake, and importantly its measurement in fasting urine samples could be used to determine chicken intake in a free-living population.
Collections Ireland -> University College Dublin -> School of Agriculture and Food Science
Ireland -> University College Dublin -> Agriculture and Food Science Research Collection
Ireland -> University College Dublin -> Institute of Food and Health
Ireland -> University College Dublin -> College of Health and Agricultural Sciences
Ireland -> University College Dublin -> Institute of Food and Health Research Collection
Ireland -> University College Dublin -> Institutes and Centres

Full list of authors on original publication

Lorraine Brennan, Michael J Gibney, Anne P Nugent, Breige A McNulty, Milena Rundle, Helena Gibbons, Xiaofei Yin

Experts in our system

1
Lorraine Brennan
University College Dublin
Total Publications: 166
 
2
Michael J Gibney
University College Dublin
Total Publications: 102
 
3
Anne P Nugent
University College Cork
 
4
Breige A McNulty
University College Cork
 
5
Milena Rundle
University College Dublin
Total Publications: 6
 
6
Helena Gibbons
University College Dublin
 
7
Xiaofei Yin
University College Dublin