Type

Journal Article

Authors

Gavin Stewart
Alan W Baird
Desmond C Winter
Hashemeya Al-mousawi
Alison McGrane
Caragh Walpole

Subjects

Microbiology

Topics
proteins ut b ut a intestine small human gastrointestinal tract humans urea

Investigation of facilitative urea transporters in the human gastrointestinal tract (2018)

Abstract The symbiotic relationship between humans and their intestinal microbiomeis supported by urea nitrogen salvaging. Previous studies have shown thatcolonic UT-B urea transporters play a significant role in this important physi-ological process. This current study investigated UT-A and UT-B urea trans-porter expression along the human gastrointestinal tract. Initial end-pointPCR experiments determined that UT-A RNA was predominantly expressedin the small intestine, while UT-B RNA was expressed in stomach, small intes-tine, and colon. Using western blotting experiments, a strong 40–60 kDa UT-B signal was found to be abundant in both ileum and colon. Importantly, thissignal was deglycosylated by PNGaseF enzyme treatment to a core protein of30 kDa in both tissues. Further immunolocalization studies revealed UT-Btransporter proteins were present at the apical membrane of the villi in theileum, but predominantly at the basolateral membrane of the colonic surfaceepithelial cells. Finally, a blind scoring immunolocalization study suggestedthat there was no significant difference in UT-B abundance throughout thecolon (NS, ANOVA,N=5–21). In conclusion, this current study suggestedUT-B to be the main human intestinal urea transporter. Intriguingly, thesedata suggested that the same UT-B isoform was present in all intestinalepithelial cells, but that the precise cellular location varied.
Collections Ireland -> University College Dublin -> College of Science
Ireland -> University College Dublin -> Conway Institute Research Collection
Ireland -> University College Dublin -> School of Biology & Environmental Science
Ireland -> University College Dublin -> Biology & Environmental Science Research Collection
Ireland -> University College Dublin -> Conway Institute
Ireland -> University College Dublin -> Institutes and Centres

Full list of authors on original publication

Gavin Stewart, Alan W Baird, Desmond C Winter, Hashemeya Al-mousawi, Alison McGrane, Caragh Walpole

Experts in our system

1
Gavin Stewart
University College Dublin
Total Publications: 7
 
2
Alan W. Baird
University College Dublin
Total Publications: 70
 
3
Desmond C. Winter
University College Dublin
Total Publications: 53
 
4
Caragh Walpole
University College Dublin
Total Publications: 3