Type

Other / n/a

Authors

Colin Hill
Julian R Marchesi
Roy D Sleator
Eamonn P Culligan

Subjects

Microbiology

Topics
food science molecular sequence data public and environmental health genetics gastrointestinal tract 3 4 health biotechnology genetics and genomics pharmacology toxicology and environmental health beta carotene 15 15 monooxygenase proteins genetics 3 medical and health sciences proteins and enzymes technologies medicine and health sciences salt tolerance cell and developmental biology cloning molecular physiology metagenomics membrane other medicine and health sciences public health microbiology biochemistry biophysics and structural biology biotechnology 3 3 health sciences biology nutrition dietetics bacteria growth development integrative and complementary medicine nutrition dna bacterial proteins sequence analysis

Metagenomic Identification of a Novel Salt Tolerance Gene from the Human Gut Microbiome Which Encodes a Membrane Protein with Homology to a brp/blh-Family beta-Carotene 15,15'-Monooxygenase (2014)

Abstract The human gut microbiome consists of at least 3 million non-redundant genes, 150 times that of the core human genome. Herein, we report the identification and characterisation of a novel stress tolerance gene from the human gut metagenome. The locus, assigned brpA, encodes a membrane protein with homology to a brp/blh-family β-carotene monooxygenase. Cloning and heterologous expression of brpA in Escherichia coli confers a significant salt tolerance phenotype. Furthermore, when cultured in the presence of exogenous β-carotene, cell pellets adopt a red/orange pigmentation indicating the incorporation of carotenoids in the cell membrane.
Collections Ireland -> Cork Institute of Technology -> Faculty of Engineering & Science
Ireland -> Cork Institute of Technology -> School of Science and Informatics
Ireland -> Cork Institute of Technology -> Department of Biological Sciences Publications
Ireland -> Cork Institute of Technology -> Faculties and Units
Ireland -> Cork Institute of Technology -> Biological Sciences

Full list of authors on original publication

Colin Hill, Julian R Marchesi, Roy D Sleator, Eamonn P Culligan

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 396
 
2
Julian R Marchesi
University College Cork
Total Publications: 45
 
3
Roy D. Sleator
Cork Institute of Technology
Total Publications: 67
 
4
Eamonn P Culligan
University College Cork
Total Publications: 17