Journal Article


David J Lynn
David E MacHugh
Daniel G Bradley
Stephen V Gordon
Kevin M. Conlon
John A Browne
Matthew S McCabe
Kenneth Bryan
Nicolas C Nalpas
David A Magee
and 1 others



alveolar macrophage tuberculosis next generation sequencing microrna host immune response mycobacterium bovis rna seq bos taurus

MicroRNA profiling of the bovine alveolar macrophage response to Mycobacterium bovis infection suggests pathogen survival is enhanced by microRNA regulation of endocytosis and lysosome trafficking (2014)

Abstract Mycobacterium bovis, the causative agent of bovine tuberculosis, a major problem for global agriculture, spreads via an airborne route and is taken up by alveolar macrophages (AM) in the lung. Here, we describe the first next-generation sequencing (RNA-seq) approach to temporally profile miRNA expression in primary bovine AMs post-infection with M. bovis. One, six, and forty miRNAs were identified as significantly differentially expressed at 2, 24 and 48 h post-infection, respectively. The differential expression of three miRNAs (bta-miR-142-5p, bta-miR-146a, and bta-miR-423-3p) was confirmed by RT-qPCR. Pathway analysis of the predicted mRNA targets of differentially expressed miRNAs suggests that these miRNAs preferentially target several pathways that are functionally relevant for mycobacterial pathogenesis, including endocytosis and lysosome trafficking, IL-1 signalling and the TGF-β pathway. Over-expression studies using a bovine macrophage cell-line (Bomac) reveal the targeting of two key genes in the innate immune response to M. bovis, IL-1 receptor-associated kinase 1 (IRAK1) and TGF-β receptor 2 (TGFBR2), by miR-146. Taken together, our study suggests that miRNAs play a key role in tuning the complex interplay between M. bovis survival strategies and the host immune response.
Collections Ireland -> Teagasc -> Animal & Bioscience
Ireland -> Teagasc -> Animal & Grassland Research & Innovation Programme

Full list of authors on original publication

David J Lynn, David E MacHugh, Daniel G Bradley, Stephen V Gordon, Kevin M. Conlon, John A Browne, Matthew S McCabe, Kenneth Bryan, Nicolas C Nalpas, David A Magee and 1 others

Experts in our system

David J Lynn
Total Publications: 37
David E. MacHugh
University College Dublin
Total Publications: 82
Daniel Bradley
Trinity College Dublin
Total Publications: 84
Stephen V. Gordon
University College Dublin
Total Publications: 40
John A Browne
University College Dublin
Total Publications: 73
Matthew S. McCabe
Total Publications: 45
Kenneth Bryan
Royal College of Surgeons in Ireland
Total Publications: 36
Nicolas C. Nalpas
University College Dublin
Total Publications: 14
David A. Magee
University College Dublin
Total Publications: 47