Type

Journal Article

Authors

David A. Kenny
M H Parr
Sinead M. Waters
Catherine Stanton
Alan K Kelly
K M Pierce
Michael G. Diskin
P Lonergan
D.N Doyle

Subjects

Veterinary

Topics
cattle embryo fatty acid aldo keto reductase gene expression progesterone embryo development n 3 pufa

Effect of dietary n-3 polyunsaturated fatty acid supplementation and post-insemination plane of nutrition on systemic concentrations of metabolic analytes, progesterone, hepatic gene expression and embryo development and survival in beef heifers (2018)

Abstract Nutrition, and particularly dietary energy intake, plays a fundamental role in reproductive function in cattle. There is some evidence that supplemental omega-3 dietary polyunsaturated fatty acids (n-3 PUFA) can exert positive effects on fertility. The objectives of this study were to evaluate the effect of dietary n-3 PUFA supplementation, post-insemination energy plane of nutrition and their interaction on embryo survival in cattle. Crossbred beef heifers (n = 185) were individually offered barley straw ad libitum and 6 kg DM of concentrate supplemented with either a rumen-protected source of saturated fatty acid (palmitic; control, CON) or a partially rumen-protected n-3 PUFA-enriched supplement (n-3 PUFA). Estrous was synchronised using two injections of PG administered at 11-d intervals and following artificial insemination (AI = Day 0) 179 heifers exhibiting oestrus were inseminated and assigned to one of two dietary treatments: (i) remain on their pre-insemination high dietary plane of nutrition (High) or (ii) restricted to 0.6 × estimated maintenance energy requirements (Low) in a 2 × 2 factorial design. The heifers were then maintained on their assigned diets until slaughter and embryo recovery on Day 16 (n = 92) or pregnancy diagnosis by ultrasound scanning at Day 30 post-AI (n = 87). Plasma concentrations of fatty acids, metabolites, insulin, progesterone (P4) and insulin-like growth factor 1 (IGF-1) were measured at appropriate intervals. Hepatic expression of mRNA for aldo-keto reductase (AKR1C), cytochrome P450 2C (CYP 2C) and cytochrome P450 3A (CYP 3A) was examined. The n-3 PUFA supplementation increased plasma n-3 PUFA concentration (P < 0.05) and reduced n-6: n-3 PUFA ratio (P < 0.05). Plasma IGF-1 was higher for n-3 PUFA relative to the CON (P < 0.05) and for High compared with Low plane of nutrition post-AI (P < 0.05) groups. A low plane of nutrition post-AI increased plasma concentrations of progesterone from Days 7–16 after insemination (P < 0.001) but reduced embryo length (P < 0.001). Supplementation with n-3 PUFA reduced and tended to reduce hepatic expression of CYP2C (P = 0.01) and CYP3A (P = 0.08), respectively. However, while dietary n-3 PUFA supplementation and an abrupt reduction in nutrient status following insemination elevated plasma concentrations of n-3 PUFA and mid and late phase P4, respectively, there was no effect of either PUFA supplementation or post-insemination plane of nutrition on embryo survival.
Collections Ireland -> Teagasc -> Food Biosciences
Ireland -> Teagasc -> Food Programme

Full list of authors on original publication

David A. Kenny, M H Parr, Sinead M. Waters, Catherine Stanton, Alan K Kelly, K M Pierce, Michael G. Diskin, P Lonergan, D.N Doyle

Experts in our system

1
D.A. Kenny
Teagasc
Total Publications: 147
 
2
M H Parr
Teagasc
Total Publications: 12
 
3
Sinead M. Waters
Teagasc
Total Publications: 99
 
4
Catherine Stanton
Teagasc
Total Publications: 314
 
5
Alan K Kelly
Teagasc
Total Publications: 88
 
6
K.M. Pierce
Teagasc
Total Publications: 36
 
7
M G Diskin
Teagasc
Total Publications: 94
 
8
P. Lonergan
University College Dublin
Total Publications: 207
 
9
D.N Doyle
Teagasc
Total Publications: 3