Type

Journal Article

Authors

Yao Chen
Shengqian Ma
Peng Cheng
Ang Li
Zhenjie Zhang
Xia Li
Yassin H. Andaloussi
Yunlong Zheng
Shan Qiao
Mingmin Li

Subjects

Chemistry

Topics
mass transfer environment enzymes capsules materials construction enzyme activity organic

Fabricating covalent organic framework capsules with commodious microenvironment for enzymes (2020)

Abstract The full text of this article will not be available in ULIR until the embargo expires on the 20/03/2021 enzyme activity is still of formidable challenge. Herein, we created a sacrificial templating method, using metal-organic frameworks (MOFs) as sacrificial templates to construct hollow covalent organic framework (COF) capsules for enzyme encapsulation. This strategy can provide capacious microenvironment to unleash enzyme molecules. The improved conformational freedom of enzymes, enhanced mass transfer and protective effect against external environment ultimately boosted the enzymatic activities. We also found that this strategy possesses high versatility that is suitable for diverse biomacromolecules, MOF templates and COF capsules. Moreover, the dimensions, pore sizes and shell thickness of COF capsules can be conveniently tuned, allowing for customizing bioreactors for specific functions. For example, coencapsulation of different enzymes with synergistic functions were successfully demonstrated using this bioreactor platform. This study not only opens up a new avenue to overcome the present limitations of enzymatic immobilization in porous matrixes but also provides new opportunities for construction of biomicrodevices or artificial organelles based on crystalline porous materials.
Collections Ireland -> University of Limerick -> Centres Science and Engineering
Ireland -> University of Limerick -> Faculty of Science and Engineering
Ireland -> University of Limerick -> Bernal Institute

Full list of authors on original publication

Yao Chen, Shengqian Ma, Peng Cheng, Ang Li, Zhenjie Zhang, Xia Li, Yassin H. Andaloussi, Yunlong Zheng, Shan Qiao, Mingmin Li

Experts in our system

1
Yao Chen
University of Limerick
Total Publications: 9
 
2
Shengqian Ma
University of Limerick
Total Publications: 4
 
3
Peng Cheng
University of Limerick
Total Publications: 14
 
4
Zhenjie Zhang
University of Limerick
Total Publications: 15
 
5
Xia Li
University of Limerick
Total Publications: 35
 
6
Yassin H. Andaloussi
University of Limerick
Total Publications: 4