Type

Other / n/a

Authors

Noel G McElvaney
Clifford C Taggart
Shane J O'Neill
Michael O'Mahony
Catherine M Greene
Patrick Geraghty

Subjects

Microbiology

Topics
nf kappa b mononuclear gene expression regulation enzymologic secretory leukocyte peptidase inhibitor chronic obstructive matrix metalloproteinase 12 leukocytes enzymologic pulmonary disease chronic obstructive pulmonary disease cations subcellular fractions medicine and health sciences u937 cells leukocytes mononuclear cathepsins interferon gamma bronchoalveolar lavage fluid humans macrophages gene expression regulation

Secretory leucocyte protease inhibitor inhibits interferon-gamma-induced cathepsin S expression. (2007)

Abstract We have demonstrated that bronchoalveolar lavage fluid from chronic obstructive pulmonary disease patients contains higher levels of interferon-gamma compared with controls. Interferon-gamma is a potent inducer of various cathepsins and matrix metalloproteases. Therefore, we postulated that interferon-gamma could induce protease expression by macrophages in acute and chronic lung disease. Chronic obstructive pulmonary disease patients had greater levels of cathepsin S and matrix metalloprotease-12 in their bronchoalveolar lavage fluid. Macrophages incubated with chronic obstructive pulmonary disease bronchoalveolar lavage fluid exhibited increased expression of cathepsin S and matrix metalloprotease-12, which was inhibited by the addition of interferon-gamma-neutralizing immunoglobulin. Human secretory leukocyte protease inhibitor is an 11.7-kDa cationic non-glycosylated antiprotease synthesized and secreted by cells at the site of inflammation. We have demonstrated that secretory leukocyte protease inhibitor can inhibit interferon-gamma-induced cathepsin S production by macrophages. Pretreatment of macrophages with secretory leukocyte protease inhibitor inhibited interferon-gamma-induced inhibitor kappaB beta degradation and activation of nuclear factor kappaB. Secretory leukocyte protease inhibitor may prove to be therapeutically important as a potential inhibitor of protease expression in chronic obstructive pulmonary disease.
Collections Ireland -> Royal College of Surgeons in Ireland -> Medicine Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Medicine

Full list of authors on original publication

Noel G McElvaney, Clifford C Taggart, Shane J O'Neill, Michael O'Mahony, Catherine M Greene, Patrick Geraghty

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
2
Clifford C Taggart
Royal College of Surgeons in Ireland
Total Publications: 54
 
3
Shane J O'Neill
Royal College of Surgeons in Ireland
Total Publications: 84
 
4
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
5
Patrick Geraghty
Royal College of Surgeons in Ireland
Total Publications: 13