Type

Other / n/a

Authors

Noel G McElvaney
Catherine M Greene
Tomás P Carroll

Subjects

Biochemistry

Topics
interleukin 8 endoplasmic reticulum unfolded protein response dna binding proteins medicine and health sciences rna messenger rna enzyme linked immunosorbent assay messenger humans transcription factors monocytes

Measurement of the unfolded protein response (UPR) in monocytes. (2011)

Abstract In mammalian cells, the primary function of the endoplasmic reticulum (ER) is to synthesize and assemble membrane and secreted proteins. As the main site of protein folding and posttranslational modification in the cell, the ER operates a highly conserved quality control system to ensure only correctly assembled proteins exit the ER and misfolded and unfolded proteins are retained for disposal. Any disruption in the equilibrium of the ER engages a multifaceted intracellular signaling pathway termed the unfolded protein response (UPR) to restore normal conditions in the cell. A variety of pathological conditions can induce activation of the UPR, including neurodegenerative disorders such as Parkinson's disease, metabolic disorders such as atherosclerosis, and conformational disorders such as cystic fibrosis. Conformational disorders are characterized by mutations that modify the final structure of a protein and any cells that express abnormal protein risk functional impairment. The monocyte is an important and long-lived immune cell and acts as a key immunological orchestrator, dictating the intensity and duration of the host immune response. Monocytes expressing misfolded or unfolded protein may exhibit UPR activation and this can compromise the host immune system. Here, we describe in detail methods and protocols for the examination of UPR activation in peripheral blood monocytes. This guide should provide new investigators to the field with a broad understanding of the tools required to investigate the UPR in the monocyte.
Collections Ireland -> Royal College of Surgeons in Ireland -> Medicine Articles
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Full list of authors on original publication

Noel G McElvaney, Catherine M Greene, Tomás P Carroll

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
2
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
3
Tomás P Carroll
Royal College of Surgeons in Ireland
Total Publications: 26