Type

Other / n/a

Authors

Jochen HM Prehn
Brian P Kirby
David C Henshall
Tobias Engel
Tytus Bernas
Hans Georg König
Eva C Bunk

Subjects

Biochemistry

Topics
apoptosis animals neurons physics cells cultured mice cell survival cells neurogenesis membrane proteins hippocampus physiology microtubule associated proteins proto oncogene proteins cultured neuropeptides phenotype tumor suppressor proteins cell differentiation apoptosis regulatory proteins bromodeoxyuridine

BH3-only proteins BIM and PUMA in the regulation of survival and neuronal differentiation of newly generated cells in the adult mouse hippocampus. (2010)

Abstract Neurogenesis persists in the adult hippocampus, where several thousand neurons are born every day. Most of the newly generated cells are eliminated by apoptosis, possibly because of their failure to integrate properly into neural networks. The BH3-only proteins Bim and Puma have been shown to mediate trophic factor withdrawal- and anoikis-induced apoptosis in various systems. We therefore determined their impact on proliferation, survival, and differentiation of adult-generated cells in the mouse hippocampus using gene-deficient mice. Wild-type, bim-, and puma-deficient mice showed similar rates of precursor cell proliferation, as evidenced by 5-bromo-2-deoxyuridine (BrdU)-incorporation. Deficiency in either bim or puma significantly increased the survival of adult-born cells in the dentate gyrus (DG) after 7 days. Consistently, we detected increased numbers of doublecortin (DCX)-positive and fewer terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelled-positive cells in the DG of bim- and puma-deficient mice. Bim and puma deficiency did not change early markers of neuronal differentiation, as evidenced by BrdU/DCX double-labelling. However, BrdU/NeuN double-labelling revealed that deficiency of bim, but not puma, accelerated the differentiation of newly generated cells into a neuronal phenotype. Our data show that Bim and Puma are prominently involved in the regulation of neuronal progenitor cell survival in the adult DG, but also suggest that Bim has an additional role in neuronal differentiation of adult-born neural precursor cells.
Collections Ireland -> Royal College of Surgeons in Ireland -> Physiology and Medical Physics Articles
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Ireland -> Royal College of Surgeons in Ireland -> Department of Physiology and Medical Physics

Full list of authors on original publication

Jochen HM Prehn, Brian P Kirby, David C Henshall, Tobias Engel, Tytus Bernas, Hans Georg König, Eva C Bunk

Experts in our system

1
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
2
Brian P Kirby
Royal College of Surgeons in Ireland
Total Publications: 48
 
3
David C Henshall
Royal College of Surgeons in Ireland
Total Publications: 127
 
4
Tobias Engel
Royal College of Surgeons in Ireland
Total Publications: 66
 
5
Tytus Bernas
Royal College of Surgeons in Ireland
Total Publications: 8
 
6
Hans Georg König
Royal College of Surgeons in Ireland
Total Publications: 22