Type

Other / n/a

Authors

David J Hughes
Jochen HM Prehn
Mazda Jenab
Massimo Tommasino
Nicki Dezeeuw
Paul Neary
Jan Bruha
Vaclav Liska
Tereza Kunicka
Pavel Soucek
and 3 others

Subjects

Medicine & Nursing

Topics
bacterial infection colorectal cancer neoplastic progression physics north america real time polymerase chain reaction physiology disease progression

Fusobacterium nucleatum associates with stages of colorectal neoplasia development, colorectal cancer and disease outcome. (2014)

Abstract Commensal bacteria in the colon may play a role in colorectal cancer (CRC) development. Recent studies from North America showed that Fusobacterium nucleatum (Fn) infection is over-represented in disease tissue versus matched normal tissue in CRC patients. Using quantitative real-time polymerase chain reaction (qPCR) of DNA extracted from colorectal tissue biopsies and surgical resections of three European cohorts totalling 122 CRC patients, we found an over-abundance of Fn in cancerous compared to matched normal tissue (p < 0.0001). To determine whether Fn infection is an early event in CRC development, we assayed Fn in colorectal adenoma (CRA) tissue from 52 Irish patients. While for all CRAs the Fn level was not statistically significantly higher in disease versus normal tissue (p = 0.06), it was significantly higher for high-grade dysplasia (p = 0.015). As a secondary objective, we determined that CRC patients with low Fn levels had a significantly longer overall survival time than patients with moderate and high levels of the bacterium (p = 0.008). The investigation of Fn as a potential non-invasive biomarker for CRC screening showed that, while Fn was more abundant in stool samples from CRC patients compared to adenomas or controls, the levels in stool did not correlate with cancer or adenoma tissue levels from the same individuals. This is the first study examining Fn in the colonic tissue and stool of European CRC and CRA patients, and suggests Fn as a novel risk factor for disease progression from adenoma to cancer, possibly affecting patient survival outcomes. Our results highlight the potential of Fn detection as a diagnostic and prognostic determinant in CRC patients.
Collections Ireland -> Royal College of Surgeons in Ireland -> Physiology and Medical Physics Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Physiology and Medical Physics

Full list of authors on original publication

David J Hughes, Jochen HM Prehn, Mazda Jenab, Massimo Tommasino, Nicki Dezeeuw, Paul Neary, Jan Bruha, Vaclav Liska, Tereza Kunicka, Pavel Soucek and 3 others

Experts in our system

1
David J Hughes
Royal College of Surgeons in Ireland
Total Publications: 24
 
2
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
3
Paul C Neary
TU Dublin (Tallaght Campus)
Total Publications: 62
 
4
Pavel Soucek
Royal College of Surgeons in Ireland
Total Publications: 6