Type

Other / n/a

Authors

Fergal J O'Brien
John P Gleeson
Amir A Al-Munajjed

Subjects

Biochemistry

Topics
tissue scaffolds collagen cattle animals extracellular matrix tomography emission computed emission computed tissue engineering feasibility studies tomography biocompatible materials calcium phosphates bone transplantation porosity bone substitutes anatomy

Development of a collagen calcium-phosphate scaffold as a novel bone graft substitute. (2008)

Abstract Previous investigations have shown that collagen shows excellent biological performance as a scaffold for tissue engineering. As a primary constituent of bone and cartilage, it demonstrates excellent cell adhesion and proliferation. However, in bone tissue engineering, it has insufficient mechanical properties for implantation in a load-bearing defect. The objective of this preliminary study was to investigate the possibility of developing a collagen/calcium-phosphate composite scaffold which would combine the biological performance and the high porosity of a collagen scaffold with the high mechanical stiffness of a calcium-phosphate scaffold. Collagen scaffolds were produced by a lyophilisation process from a collagen slurry. The scaffolds were soaked for different exposure times in solutions of 0.1 M, 0.5 M or 1.0 M NaNH4HPO4 followed by 0.1 M, 0.5 M or 1.0 M CaCl2. Mechanical tests of each scaffold were performed on a uniaxial testing system. Young's moduli were determined from stress-strain curves. The pore structure and porosity of the scaffolds were investigated using micro-computed tomography. A pure collagen scaffold served as a control. All scaffolds showed a significantly increased compressive stiffness relative to the pure collagen scaffolds. The exposure to the 0.5 M solutions showed significantly superior results compared to the other groups. Analysis of the pore structure indicated a decrease in the overall porosity of the composite scaffolds relative to the controls. Regarding mechanical stiffness and porosity, scaffolds after 1 hour exposure to the 0.5 M solutions showed the best properties for bone tissue engineering. Further work will involve producing a scaffold with a more homogeneous calcium phosphate distribution.
Collections Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Anatomy

Full list of authors on original publication

Fergal J O'Brien, John P Gleeson, Amir A Al-Munajjed

Experts in our system

1
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
 
2
John P Gleeson
Royal College of Surgeons in Ireland
Total Publications: 26