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Fergal J O'Brien
Daniel J Kelly
Niamh A Plunkett
Sonia Partap



collagen glycosaminoglycan scaffolds osteoblasts perfusion bioreactor bioreactor rest culture anatomy collagen glycosaminoglycan cg scaffolds

Stimulation of osteoblasts using rest periods during bioreactor culture on collagen-glycosaminoglycan scaffolds. (2009)

Abstract Osteoblasts respond to mechanical signals which play a key role in the formation of bone however, after extended periods of stimulation they become desensitised. Mechanosensitivity has been shown to be restored by the introduction of resting periods between loadings. The aim of this study was to analyse the effect of rest periods on the response of osteoblast-like cells seeded on collagen-glycosaminoglycan (CG) scaffolds in a flow perfusion bioreactor up to 14 days. Short (10 s) and long (7 h) term rests were incorporated into stimulation patterns. Constructs cultured in the bioreactor had a more homogenous cell distribution albeit with lower cell numbers than the static group. Osteopontin expression was significantly higher on the rest-inserted group than on the steady flow and static control. These results indicate that the insertion of short term rests during flow improves cellular distribution and osteogenic responses on CG constructs cultured in a flow perfusion bioreactor.
Collections Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Anatomy

Full list of authors on original publication

Fergal J O'Brien, Daniel J Kelly, Niamh A Plunkett, Sonia Partap

Experts in our system

Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 268
Daniel Kelly
Trinity College Dublin
Total Publications: 190
Sonia Partap
Royal College of Surgeons in Ireland
Total Publications: 11