Type

Other / n/a

Authors

Brian C O'Connell
Patrick J Prendergast
Veronica A Campbell
Fergal J O'Brien
Matthew G Haugh
Louise A McMahon
Eric Farrell
Elaine M Byrne

Subjects

Biochemistry

Topics
anatomy time factors cultured gene expression cells wistar materials testing cell differentiation animals tissue scaffolds rats stromal cells rats wistar cell culture techniques bone marrow cells glycosaminoglycans rna collagen type i cells cultured biomechanics porosity

Gene expression by marrow stromal cells in a porous collagen-glycosaminoglycan scaffold is affected by pore size and mechanical stimulation. (2008)

Abstract Marrow stromal cell (MSC) populations, which are a potential source of undifferentiated mesenchymal cells, and culture scaffolds that mimic natural extracellular matrix are attractive options for orthopaedic tissue engineering. A type I collagen-glycosaminoglycan (CG) scaffold that has previously been used clinically for skin regeneration was recently shown to support expression of bone-associated proteins and mineralisation by MSCs cultured in the presence of osteogenic supplements. Here we follow RNA markers of osteogenic differentiation in this scaffold. We demonstrate that transcripts of the late stage markers bone sialoprotein and osteocalcin are present at higher levels in scaffold constructs than in two-dimensional culture, and that considerable gene induction can occur in this scaffold even in the absence of soluble osteogenic supplements. We also find that bone-related gene expression is affected by pore size, mechanical constraint, and uniaxial cyclic strain of the CG scaffold. The data presented here further establish the CG scaffold as a potentially valuable substrate for orthopaedic tissue engineering and for research on the mechanical interactions between cells and their environment, and suggest that a more freely-contracting scaffold with larger pore size may provide an environment more conducive to osteogenesis than constrained scaffolds with smaller pore sizes.
Collections Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Anatomy

Full list of authors on original publication

Brian C O'Connell, Patrick J Prendergast, Veronica A Campbell, Fergal J O'Brien, Matthew G Haugh, Louise A McMahon, Eric Farrell, Elaine M Byrne

Experts in our system

1
Patrick John Prendergast
Trinity College Dublin
Total Publications: 59
 
2
Veronica Campbell
Trinity College Dublin
Total Publications: 19
 
3
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
 
4
Matthew G Haugh
Royal College of Surgeons in Ireland
Total Publications: 20
 
5
Eric Farrell
Royal College of Surgeons in Ireland
Total Publications: 9