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Daniel J Kelly
Veronica A Campbell
Fergal J O'Brien
Tatiana Vinardell
Conor T Buckley
Stephen D Thorpe



bioreactor dynamic compression functional tissue engineering transforming growth factor beta3 mechanobiology cartilage mesenchymal stem cells anatomy

The response of bone marrow-derived mesenchymal stem cells to dynamic compression following TGF-beta3 induced chondrogenic differentiation. (2010)

Abstract The objective of this study was to investigate the hypothesis that the application of dynamic compression following transforming growth factor-beta3 (TGF-beta3) induced differentiation will further enhance chondrogenesis of mesenchymal stem cells (MSCs). Porcine MSCs were encapsulated in agarose hydrogels and cultured in a chemically defined medium with TGF-beta3 (10 ng/mL). Dynamic compression (1 Hz, 10% strain, 1 h/day) was initiated at either day 0 or day 21 and continued until day 42 of culture; with TGF-beta3 withdrawn from some groups at day 21. Biochemical and mechanical properties of the MSC-seeded constructs were evaluated up to day 42. The application of dynamic compression from day 0 inhibited chondrogenesis of MSCs. This inhibition of chondrogenesis in response to dynamic compression was not observed if MSC-seeded constructs first underwent 21 days of chondrogenic differentiation in the presence of TGF-beta3. Spatial differences in sGAG accumulation in response to both TGF-beta3 stimulation and dynamic compression were observed within the constructs. sGAG release from the engineered construct into the surrounding culture media was also dependent on TGF-beta3 stimulation, but was not effected by dynamic compression. Continued supplementation with TGF-beta3 appeared to be a more potent chondrogenic stimulus than the application of 1 h of daily dynamic compression following cytokine initiated differentiation. In the context of cartilage tissue engineering, the results of this study suggest that MSC seeded constructs should be first allowed to undergo chondrogenesis in vitro prior to implantation in a load bearing environment.
Collections Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Anatomy

Full list of authors on original publication

Daniel J Kelly, Veronica A Campbell, Fergal J O'Brien, Tatiana Vinardell, Conor T Buckley, Stephen D Thorpe

Experts in our system

Daniel Kelly
Trinity College Dublin
Total Publications: 168
Veronica Campbell
Trinity College Dublin
Total Publications: 19
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
Tatiana Vinardell
Trinity College Dublin
Total Publications: 27
Conor T Buckley
Trinity College Dublin
Total Publications: 85
Stephen D Thorpe
Trinity College Dublin
Total Publications: 18