Type

Other / n/a

Authors

Sally-Ann Cryan
Fergal O'Brien
Rosanne Raftery

Subjects

Biochemistry

Topics
gene activated matrices tissue engineering non viral gene delivery gene therapy anatomy chitosan pdna sirna

Chitosan for gene delivery and orthopedic tissue engineering applications. (2013)

Abstract Gene therapy involves the introduction of foreign genetic material into cells in order exert a therapeutic effect. The application of gene therapy to the field of orthopaedic tissue engineering is extremely promising as the controlled release of therapeutic proteins such as bone morphogenetic proteins have been shown to stimulate bone repair. However, there are a number of drawbacks associated with viral and synthetic non-viral gene delivery approaches. One natural polymer which has generated interest as a gene delivery vector is chitosan. Chitosan is biodegradable, biocompatible and non-toxic. Much of the appeal of chitosan is due to the presence of primary amine groups in its repeating units which become protonated in acidic conditions. This property makes it a promising candidate for non-viral gene delivery. Chitosan-based vectors have been shown to transfect a number of cell types including human embryonic kidney cells (HEK293) and human cervical cancer cells (HeLa). Aside from its use in gene delivery, chitosan possesses a range of properties that show promise in tissue engineering applications; it is biodegradable, biocompatible, has anti-bacterial activity, and, its cationic nature allows for electrostatic interaction with glycosaminoglycans and other proteoglycans. It can be used to make nano- and microparticles, sponges, gels, membranes and porous scaffolds. Chitosan has also been shown to enhance mineral deposition during osteogenic differentiation of MSCs in vitro. The purpose of this review is to critically discuss the use of chitosan as a gene delivery vector with emphasis on its application in orthopedic tissue engineering.
Collections Ireland -> Royal College of Surgeons in Ireland -> School of Pharmacy
Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
Ireland -> Royal College of Surgeons in Ireland -> School of Pharmacy Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Anatomy

Full list of authors on original publication

Sally-Ann Cryan, Fergal O'Brien, Rosanne Raftery

Experts in our system

1
Sally-Ann Cryan
Royal College of Surgeons in Ireland
Total Publications: 67
 
2
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
 
3
Rosanne Raftery
Royal College of Surgeons in Ireland
Total Publications: 9