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Fergal O'Brien
C Jungreuthmayer
Ryan J McCoy



shear stress perfusion bioreactor cell size anatomy cell detachment pore size finite elements analysis computational fluid dynamics

Influence of flow rate and scaffold pore size on cell behavior during mechanical stimulation in a flow perfusion bioreactor. (2012)

Abstract Mechanically stimulating cell-seeded scaffolds by flow-perfusion is one approach utilized for developing clinically applicable bone graft substitutes. A key challenge is determining the magnitude of stimuli to apply that enhances cell differentiation but minimizes cell detachment from the scaffold. In this study, we employed a combined computational modeling and experimental approach to examine how the scaffold mean pore size influences cell attachment morphology and subsequently impacts upon cell deformation and detachment when subjected to fluid-flow. Cell detachment from osteoblast-seeded collagen-GAG scaffolds was evaluated experimentally across a range of scaffold pore sizes subjected to different flow rates and exposure times in a perfusion bioreactor. Cell detachment was found to be proportional to flow rate and inversely proportional to pore size. Using this data, a theoretical model was derived that accurately predicted cell detachment as a function of mean shear stress, mean pore size, and time. Computational modeling of cell deformation in response to fluid flow showed the percentage of cells exceeding a critical threshold of deformation correlated with cell detachment experimentally and the majority of these cells were of a bridging morphology (cells stretched across pores). These findings will help researchers optimize the mean pore size of scaffolds and perfusion bioreactor operating conditions to manage cell detachment when mechanically simulating cells via flow perfusion. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc.
Collections Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
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Full list of authors on original publication

Fergal O'Brien, C Jungreuthmayer, Ryan J McCoy

Experts in our system

Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
Ryan J McCoy
Royal College of Surgeons in Ireland