Type

Other / n/a

Authors

Wolfgang Gowin
Michael Giehl
Fergal J O'Brien
TThorfinnur Gunnlaugsson
Raman Parkesh
David Taylor
Sahar Mohsin
T Clive Lee

Subjects

Biochemistry

Topics
microscopy fluorescence anti bacterial agents tetracyclines rosaniline dyes contrast media bone and bones fluorescence x ray computed animals tomography x ray computed osteoporosis fractures fluorescent dyes tomography fractures stress microscopy confocal chelating agents anatomy stress confocal humans microscopy

Detecting microdamage in bone. (2003)

Abstract Fatigue-induced microdamage in bone contributes to stress and fragility fractures and acts as a stimulus for bone remodelling. Detecting such microdamage is difficult as pre-existing microdamage sustained in vivo must be differentiated from artefactual damage incurred during specimen preparation. This was addressed by bulk staining specimens in alcohol-soluble basic fuchsin dye, but cutting and grinding them in an aqueous medium. Nonetheless, some artefactual cracks are partially stained and careful observation under transmitted light, or epifluorescence microscopy, is required. Fuchsin lodges in cracks, but is not site-specific. Cracks are discontinuities in the calcium-rich bone matrix and chelating agents, which bind calcium, can selectively label them. Oxytetracycline, alizarin complexone, calcein, calcein blue and xylenol orange all selectively bind microcracks and, as they fluoresce at different wavelengths and colours, can be used in sequence to label microcrack growth. New agents that only fluoresce when involved in a chelate are currently being developed--fluorescent photoinduced electron transfer (PET) sensors. Such agents enable microdamage to be quantified and crack growth to be measured and are useful histological tools in providing data for modelling the material behaviour of bone. However, a non-invasive method is needed to measure microdamage in patients. Micro-CT is being studied and initial work with iodine dyes linked to a chelating group has shown some promise. In the long term, it is hoped that repeated measurements can be made at critical sites and microdamage accumulation monitored. Quantification of microdamage, together with bone mass measurements, will help in predicting and preventing bone fracture failure in patients with osteoporosis.
Collections Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Anatomy

Full list of authors on original publication

Wolfgang Gowin, Michael Giehl, Fergal J O'Brien, TThorfinnur Gunnlaugsson, Raman Parkesh, David Taylor, Sahar Mohsin, T Clive Lee

Experts in our system

1
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
 
2
David Taylor
Trinity College Dublin
Total Publications: 75
 
3
T Clive Lee
Royal College of Surgeons in Ireland
Total Publications: 76