Type

Other / n/a

Authors

Fergal O'Brien
Nehar Celikkin
Elaine Quinlan
Adolfo López-Noriega

Subjects

Engineering

Topics
bone tissue engineering peptide engineering anatomy tissue engineering scaffolds collagen scaffolds pro osteogenic peptide polymeric microparticles collagen hydroxyapatite

Incorporation of polymeric microparticles into collagen-hydroxyapatite scaffolds for the delivery of a pro-osteogenic peptide for bone tissue engineering (2015)

Abstract Collagen-hydroxyapatite scaffolds are outstanding materials for bone tissue engineering as they are biocompatible, bioresorbable, osteoconductive, and osteoinductive. The objective of the present work was to assess the potential of increasing their regenerative capacity by functionalising the scaffolds for therapeutic delivery. This was achieved by the utilization ofpolymeric drug carriers. With this purpose, alginate, chitosan, gelatine, and poly(lactic-co-glycolic acid) (PLGA) microparticles eluting PTHrP 107-111, an osteogenic pentapeptide, werefabricated and tested by incorporating them into the scaffolds. Among them, PLGA microparticles show the most promising characteristics for use as drug delivery devices. Following the incorporation of the microparticles, the scaffolds maintained their interconnected porous structure and the mechanical properties of the materials were not adversely affected. In addition, the microparticles released all their PTHrP 107-111 cargo. Most importantly, the delivered peptide proved to be bioactive and promoted enhanced osteogenesis as assessed by alkaline phosphatase production and osteocalcin and osteopontin gene expression when pre-osteoblastic cells were seeded on the scaffolds. While the focus was on bone repair, the release system described in this study can be used for the delivery of therapeutics for healing and regeneration of a variety of tissue types depending on the type of collagen scaffold chosen.
Collections Ireland -> Royal College of Surgeons in Ireland -> School of Pharmacy
Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
Ireland -> Royal College of Surgeons in Ireland -> School of Pharmacy Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Anatomy

Full list of authors on original publication

Fergal O'Brien, Nehar Celikkin, Elaine Quinlan, Adolfo López-Noriega

Experts in our system

1
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
 
2
Elaine Quinlan
Royal College of Surgeons in Ireland
Total Publications: 11
 
3
Adolfo López-Noriega
Royal College of Surgeons in Ireland
Total Publications: 10