Type

Other / n/a

Authors

Fergal O'Brien
Sally-Ann Cryan
Helena M Kelly
Emmet Thompson
Adolfo López-Noriega
Elaine Quinlan

Subjects

Engineering

Topics
rhbmp 2 plga microparticles calcium alginate collagen based scaffolds bone tissue engineering tissue engineering scaffolds anatomy

Development of collagen-hydroxyapatite scaffolds incorporating PLGA and alginate microparticles for the controlled delivery of rhBMP-2 for bone tissue engineering. (2015)

Abstract The spatiotemporally controlled delivery of the pro-osteogenic factor rhBMP-2 would overcome most of the severe secondary effects linked to the products delivering this protein for bone regeneration. With this in mind, the aim of the present work was to develop a controlled rhBMP-2 release system using collagen-hydroxyapatite (CHA) scaffolds, which had been previously optimized for bone regeneration, as delivery platforms to produce a device with enhanced capacity for bone repair. Spray-drying and emulsion techniques were used to encapsulate bioactive rhBMP-2 in alginate and PLGA microparticles, with a high encapsulation efficiency. After incorporation of these microparticles into the scaffolds, rhBMP-2 was delivered in a sustained fashion for up to 28days. When tested in vitro with osteoblasts, these eluting materials showed an enhanced pro-osteogenic effect. From these results, an optimal rhBMP-2 eluting scaffold composition was selected and implanted in critical-sized calvarial defects in a rat model, where it demonstrated an excellent healing capacity in vivo. These platforms have an immense potential in the field of tissue regeneration; by tuning the specific therapeutic molecule to the tissue of interest and by utilizing different collagen-based scaffolds, similar systems can be developed for enhancing the healing of a diverse range of tissues and organs.
Collections Ireland -> Royal College of Surgeons in Ireland -> School of Pharmacy
Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
Ireland -> Royal College of Surgeons in Ireland -> School of Pharmacy Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Anatomy

Full list of authors on original publication

Fergal O'Brien, Sally-Ann Cryan, Helena M Kelly, Emmet Thompson, Adolfo López-Noriega, Elaine Quinlan

Experts in our system

1
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 268
 
2
Sally-Ann Cryan
Royal College of Surgeons in Ireland
Total Publications: 67
 
3
Helena M Kelly
Royal College of Surgeons in Ireland
 
4
Emmet M Thompson
Royal College of Surgeons in Ireland
Total Publications: 17
 
5
Adolfo López-Noriega
Royal College of Surgeons in Ireland
Total Publications: 10
 
6
Elaine Quinlan
Royal College of Surgeons in Ireland
Total Publications: 11