Type

Other / n/a

Authors

Dermot Kenny
Jochen HM Prehn
B MacCraith
Derek Murphy
Robert Cummins
Frank E Murray
Elaine W Kay
Suzanne Hector
Gregor Kijanka

Subjects

Medicine & Nursing

Topics
aged life sciences tumor markers middle aged metabolism colorectal neoplasms prospective studies neoplasm humans antigens female immunology antibodies diagnosis oligonucleotide array sequence analysis method gene expression profiling methods colonoscopy neoplasm blood immunoglobulin g methods male biological blood reverse transcriptase polymerase chain reaction methods intestinal mucosa

Human IgG antibody profiles differentiate between symptomatic patients with and without colorectal cancer (2010)

Abstract Abstract OBJECTIVE: Patients with cancer have antibodies against tumour antigens. Characterising the antibody repertoire may provide insights into aberrant cellular mechanisms in cancer development, ultimately leading to novel diagnostic or therapeutic targets. The aim of this study was to characterise the antibody profiles in patients whose symptoms warranted colonoscopy, to see if there was a difference in patients with and without colorectal cancer.METHODS: Patients were recruited from a colonoscopy clinic. Individual serum samples from 43 patients with colorectal cancer and 40 patients with no cancer on colonoscopy were profiled on a 37 830 clone recombinant human protein array. Antigen expression was evaluated by quantitative reverse transcription-PCR and by immunohistochemistry on tissue microarrays.RESULTS: Using a sex- and age-matched training set, 18 antigens associated with cancer and 4 associated with the absence of cancer (p<0.05) were identified and confirmed. To investigate the mechanisms triggering antibody responses to these antigens, antigen expression was examined in normal colorectal mucosa and colorectal carcinoma of the same patients. The identified antigens showed cellular accumulation (p53), aberrant cellular expression (high mobility group B1 (HMGB1)) and overexpression (tripartite motif-containing 28 (TRIM28), p53, HMGB1, transcription factor 3 (TCF3), longevity assurance gene homologue 5 (LASS5) and zinc finger protein 346 (ZNF346)) in colorectal cancer tissue compared with normal colorectal mucosa.CONCLUSIONS: It is demonstrated for the first time that screening high-density protein arrays identifies unique antibody profiles that discriminate between symptomatic patients with and without colorectal cancer. The differential expression of identified antigens suggests their involvement in aberrant cellular mechanisms in cancer
Collections Ireland -> Royal College of Surgeons in Ireland -> Department of Molecular and Cellular Therapeutics
Ireland -> Royal College of Surgeons in Ireland -> Molecular and Cellular Therapeutics Articles

Full list of authors on original publication

Dermot Kenny, Jochen HM Prehn, B MacCraith, Derek Murphy, Robert Cummins, Frank E Murray, Elaine W Kay, Suzanne Hector, Gregor Kijanka

Experts in our system

1
Dermot Kenny
Royal College of Surgeons in Ireland
 
2
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
3
Robert Cummins
Royal College of Surgeons in Ireland
Total Publications: 22
 
4
F E Murray
Royal College of Surgeons in Ireland
 
5
Elaine W Kay
Royal College of Surgeons in Ireland
Total Publications: 157
 
6
Suzanne Hector
Royal College of Surgeons in Ireland
Total Publications: 13
 
7
Gregor Kijanka
Dublin City University