Type

Other / n/a

Authors

Raymond L Stallings
Maureen O'Sullivan
Anne O'Meara
Desmond G Higgins
Patrick W McGrady
Wendy B London
Frank Speleman
Jo Vandesompele
Pieter Mestdagh
Leah Alcock
and 6 others

Subjects

Biochemistry

Topics
humans sequence analysis neoplastic dna micrornas metabolism sympathetic nervous system pathology gene expression regulation chromosome aberrations cluster analysis reverse transcriptase polymerase chain reaction nervous system neoplasms genetics metabolism nuclear proteins biosynthesis genetics nucleic acid hybridization neuroblastoma genetics oncogene proteins biosynthesis genetics genetics cohort studies treatment outcome tumor medicine and health sciences cell line sensitivity and specificity

Widespread dysregulation of MiRNAs by MYCN amplification and chromosomal imbalances in neuroblastoma: association of miRNA expression with survival. (2009)

Abstract MiRNAs regulate gene expression at a post-transcriptional level and their dysregulation can play major roles in the pathogenesis of many different forms of cancer, including neuroblastoma, an often fatal paediatric cancer originating from precursor cells of the sympathetic nervous system. We have analyzed a set of neuroblastoma (n = 145) that is broadly representative of the genetic subtypes of this disease for miRNA expression (430 loci by stem-loop RT qPCR) and for DNA copy number alterations (array CGH) to assess miRNA involvement in disease pathogenesis. The tumors were stratified and then randomly split into a training set (n = 96) and a validation set (n = 49) for data analysis. Thirty-seven miRNAs were significantly over- or under-expressed in MYCN amplified tumors relative to MYCN single copy tumors, indicating a potential role for the MYCN transcription factor in either the direct or indirect dysregulation of these loci. In addition, we also determined that there was a highly significant correlation between miRNA expression levels and DNA copy number, indicating a role for large-scale genomic imbalances in the dysregulation of miRNA expression. In order to directly assess whether miRNA expression was predictive of clinical outcome, we used the Random Forest classifier to identify miRNAs that were most significantly associated with poor overall patient survival and developed a 15 miRNA signature that was predictive of overall survival with 72.7% sensitivity and 86.5% specificity in the validation set of tumors. We conclude that there is widespread dysregulation of miRNA expression in neuroblastoma tumors caused by both over-expression of the MYCN transcription factor and by large-scale chromosomal imbalances. MiRNA expression patterns are also predicative of clinical outcome, highlighting the potential for miRNA mediated diagnostics and therapeutics.
Collections Ireland -> Royal College of Surgeons in Ireland -> Cancer Genetics Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Cancer Genetics

Full list of authors on original publication

Raymond L Stallings, Maureen O'Sullivan, Anne O'Meara, Desmond G Higgins, Patrick W McGrady, Wendy B London, Frank Speleman, Jo Vandesompele, Pieter Mestdagh, Leah Alcock and 6 others

Experts in our system

1
Raymond L Stallings
Royal College of Surgeons in Ireland
Total Publications: 59
 
2
Jo Vandesompele
Royal College of Surgeons in Ireland
Total Publications: 9